FRET spectroscopy—towards effective biomolecular probing

The advent of super-resolution microscopy has been a major breakthrough in bioscience research, allowing accurate molecular signaling analysis of cellular and biological materials. Förster resonance energy transfer (FRET) spectroscopy, for instance, has emerged to be significant over the past few de...

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Main Authors: Eugene Marfo Obeng, Elvina Clarie Dullah, Michael K. Danquah, Cahyo Budiman, Clarence M. Ongkudon
Format: Article
Language:English
Published: The Royal Society of Chemistry 2016
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Online Access:https://eprints.ums.edu.my/id/eprint/19897/1/FRET%20spectroscopy.pdf
https://eprints.ums.edu.my/id/eprint/19897/
http://doi.org/10.1039/C6AY00950F
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Institution: Universiti Malaysia Sabah
Language: English
id my.ums.eprints.19897
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spelling my.ums.eprints.198972018-04-19T03:30:15Z https://eprints.ums.edu.my/id/eprint/19897/ FRET spectroscopy—towards effective biomolecular probing Eugene Marfo Obeng Elvina Clarie Dullah Michael K. Danquah Cahyo Budiman Clarence M. Ongkudon QD Chemistry The advent of super-resolution microscopy has been a major breakthrough in bioscience research, allowing accurate molecular signaling analysis of cellular and biological materials. Förster resonance energy transfer (FRET) spectroscopy, for instance, has emerged to be significant over the past few decades, owing to its non-invasive spatiotemporal cellular and subcellular probing abilities. The success of FRET has been the resurgence of fluorescent proteins (FPs) and sophisticated imaging techniques. The developments in FP and FRET-based techniques have made FRET investigations possible in diverse biotechnological fields. However, the current literature suffers a dearth in terms of a review that explains the fundamental principles (with examples) of the major areas of FRET application. This article presents a retrospective overview of the salient exploits and advancements of FRET spectroscopy and discusses the current challenges with some options. Moreover, some of the much anticipated future applications have been highlighted. The Royal Society of Chemistry 2016 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19897/1/FRET%20spectroscopy.pdf Eugene Marfo Obeng and Elvina Clarie Dullah and Michael K. Danquah and Cahyo Budiman and Clarence M. Ongkudon (2016) FRET spectroscopy—towards effective biomolecular probing. Analytical Methods, 8 (27). pp. 5323-5337. ISSN 1759-9660 http://doi.org/10.1039/C6AY00950F
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Eugene Marfo Obeng
Elvina Clarie Dullah
Michael K. Danquah
Cahyo Budiman
Clarence M. Ongkudon
FRET spectroscopy—towards effective biomolecular probing
description The advent of super-resolution microscopy has been a major breakthrough in bioscience research, allowing accurate molecular signaling analysis of cellular and biological materials. Förster resonance energy transfer (FRET) spectroscopy, for instance, has emerged to be significant over the past few decades, owing to its non-invasive spatiotemporal cellular and subcellular probing abilities. The success of FRET has been the resurgence of fluorescent proteins (FPs) and sophisticated imaging techniques. The developments in FP and FRET-based techniques have made FRET investigations possible in diverse biotechnological fields. However, the current literature suffers a dearth in terms of a review that explains the fundamental principles (with examples) of the major areas of FRET application. This article presents a retrospective overview of the salient exploits and advancements of FRET spectroscopy and discusses the current challenges with some options. Moreover, some of the much anticipated future applications have been highlighted.
format Article
author Eugene Marfo Obeng
Elvina Clarie Dullah
Michael K. Danquah
Cahyo Budiman
Clarence M. Ongkudon
author_facet Eugene Marfo Obeng
Elvina Clarie Dullah
Michael K. Danquah
Cahyo Budiman
Clarence M. Ongkudon
author_sort Eugene Marfo Obeng
title FRET spectroscopy—towards effective biomolecular probing
title_short FRET spectroscopy—towards effective biomolecular probing
title_full FRET spectroscopy—towards effective biomolecular probing
title_fullStr FRET spectroscopy—towards effective biomolecular probing
title_full_unstemmed FRET spectroscopy—towards effective biomolecular probing
title_sort fret spectroscopy—towards effective biomolecular probing
publisher The Royal Society of Chemistry
publishDate 2016
url https://eprints.ums.edu.my/id/eprint/19897/1/FRET%20spectroscopy.pdf
https://eprints.ums.edu.my/id/eprint/19897/
http://doi.org/10.1039/C6AY00950F
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