Removal of endotoxins from plasmid DNA: analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA

• Main Authors: Clarence M. Ongkudon, Hodges E, Murphy K, Danquah MK
• Format: Article
• Language: English
• Published: John Wiley & Sons Ltd. 2012
• Subjects: QH Natural history
• Online Access: https://eprints.ums.edu.my/id/eprint/20417/1/Removal%20of%20endotoxins%20from%20plasmid%20DNA.pdf; https://eprints.ums.edu.my/id/eprint/20417/; https://doi.org/10.1002/jssc.201200237
• Institution: Universiti Malaysia Sabah

Endotoxin lipopolysaccharide removal from plasmid DNA-based vaccine remains a very challenging task for bioprocess engineers. This paper examined the potential use and advantages of divalent cation (Zn(2+), Ca(2+), Mg(2+)) induced aggregation as a plasmid DNA purification method for lipopolysacchari...