Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves

• Main Authors: Mohammad Shaheen Khan, Samina Khan Yusufzai, Lawrance Kimin, Noor Adzianni Nabila Binti Jabi
• Format: Article
• Language: English
• Published: 2018
• Subjects: QK Botany
• Online Access: https://eprints.ums.edu.my/id/eprint/22901/1/Determination%20of%20Chemical%20Composition%2C%20Total%20Flavonoid%20Content%2C%20Total%20Phenolic%20Content%20and%20Antioxidant%20Capacity%20of%20Various%20Crude%20Extracts%20of%20Manihot%20esculenta%20Crantz%20Leaves.pdf; https://eprints.ums.edu.my/id/eprint/22901/
• Institution: Universiti Malaysia Sabah

In the present study, the leaves of Manihot esculenta Crantz were extracted by various organic solvents viz. hexane, chloroform, ethyl acetate and subjected to GC-MS analysis to determine their chemical composition and antioxidant activity by DPPH assay. The GC-MS analysis of hexane extract displayed squalene (10.25%), 9-octadecenoic acid (Z)-, methyl ester (9.01%), phytol (8.71%) and hexadecanoic acid, methyl ester (8.33%) as major compounds. The major compounds present in the chloroform extract were β-amyrin (12.70%), phytol (11.35%), 1,4-methanoazulen-9-ol, decahydro-1,5,5,8atetramethyl-,[1R- (1α,3aβ,4α,8aβ,9S*)]- (10.98%), 6β-Bicyclo[4.3.0]nonane, 5β-iodomethyl-1β-isopropenyl-4α,5α-dimethyl-, (9.40%) and humulane-1,6-dien-3-ol (8.53%). In ethyl acetate extract, squalene (16.39%), 9,11-dimethyltetracyclo [7.3.1.0(2.7).1(7.11)]tetradecane (12.01%) and humulane-1,6-dien-3-ol (9.58%) were reported as major compounds. The total phenolic content for hexane, chloroform and ethyl acetate was 5.35 ± 0.66 mg GAE/g, 7.52 ± 0.09 mg GAE/g and 13.47 ± 0.56 mg GAE/g, respectively. Whereas the total flavonoid content for hexane, chloroform and ethyl acetate was 0.89 ± 0.17 mg QE/g, 2.69 ± 0.09 mg QE/g and 6.66 ± 0.94 mg QE/g, respectively. The antioxidant activity of ethyl acetate extract (IC50 = 0.19 mg/mL) was higher than chloroform (IC50 = 1.39 mg/mL) and hexane extracts (IC50 = 1.74 mg/mL), with respect to the standard butylatedhydroxytoluene (BHT) (IC50 = 0.04 mg/mL).