New flavonoid chemotypes from Asplenium normale (Aspleniaceae) in Malaysia

• Main Authors: Tsukasa Iwashina, Sadamu Matsumoto, Takehisa Nakamura, Goro Kokubugata, Monica Suleiman, Idoris M. Said
• Format: Article
• Language: English; English
• Published: Kokuritsu Kagaku Hakubutsukan, Tokyo 2008
• Subjects: QH Natural history; QK Botany
• Online Access: https://eprints.ums.edu.my/id/eprint/27569/1/New%20flavonoid%20chemotypes%20from%20Asplenium%20normale%20%28Aspleniaceae%29%20in%20Malaysia%20ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/27569/2/New%20flavonoid%20chemotypes%20from%20Asplenium%20normale%20%28Aspleniaceae%29%20in%20Malaysia%20FULL%20TEXT.pdf; https://eprints.ums.edu.my/id/eprint/27569/; https://www.kahaku.go.jp/research/publication/memoir/download/45/4511.pdf
• Institution: Universiti Malaysia Sabah

Seven Asplenium normale individuals in Malaysia were surveyed for flavonoid com¬pounds. They were divided into two chemotypes, H- and I-types. The flavonoids were isolated by various chromatography and identified by TLC, HPLC, UV spectroscopic, LC-MS and NMR sur¬veys. Two flavone O-glycosides, apigenin 7-O-rhamnosyl-(1→4)-rhamnoside (1) and apigenin 7- O-rhamnosyl-(1→4)-rhamnoside-4'-O-rhamnoside (2), and two flavone C-glycosides, vicenin-2 (6) and lucenin-2 (7), were contained in one chemotype (H-type). On the other hand, two flavonol O-glycosides, kaempferol 3-O-glucosylrhamnoside (3) and kaempferol 3,4'-di-O-glycoside (4) and a flavone O-glycoside, genkwanin 4'-O-glucosyl-(1→3)-rhamnoside (5), were found from another chemotype (I-type) together with 6 and 7. In cases of Japanese Asplenium normale and related species, seven chemotypes have been reported. However, their chemotypes did not include flavonol O-glycosides and apigenin trirhamnoside. Apigenin 7-O-rhamnosyl-(1→4)-rhamnoside (1) and apigenin 7-O-rhamnosyl-(1→4)-rhamnoside-4'-O-rhamnoside (2) were reported in nature for the first time.