Total flavonoid content and antioxidant activity by different drying and extraction methods of Clinacanthus nutans leaves

• Main Authors: Mohd Hafiz Abdul Majid, Yvonne Melse Laurence, Mohammad Shaheen Khan, Mohd Sani Sarjadi
• Format: Article
• Language: English; English
• Published: Universiti Malaysia Sabah 2018
• Subjects: SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants; T1-995 Technology (General)
• Online Access: https://eprints.ums.edu.my/id/eprint/30483/1/Total%20flavonoid%20content%20and%20antioxidant%20activity%20by%20different%20drying%20and%20extraction%20methods%20of%20Clinacanthus%20nutans%20leaves.pdf; https://eprints.ums.edu.my/id/eprint/30483/2/Total%20flavonoid%20content%20and%20antioxidant%20activity%20by%20different%20drying%20and%20extraction%20methods%20of%20Clinacanthus%20nutans%20leaves1.pdf; https://eprints.ums.edu.my/id/eprint/30483/; http://borneoscience.ums.edu.my/wp-content/uploads/2018/09/2-TOTAL-FLAVONOID-CONTENT-AND-ANTIOXIDANT-ACTIVITYBY-DIFFERENT-DRYINGAND-EXTRACTION-METHODS-OF-Clinacanthus-nutans-LEAVES-new-1.pdf
• Institution: Universiti Malaysia Sabah

This report presents a study on the total flavonoid content and antioxidant activity of Clinacanthus nutans leaves from different drying and extraction methods. The C. nutans leaves were subjected through three different drying methods: oven (40°C, 60°C and 80°C), vacuum oven and air drying. Afterwards, extraction on the dried leaves was performed using three different extraction techniques: Soxhlet, maceration and ultrasound assisted solvent extraction. Finally, total flavonoid and antioxidant activity was determined spectrophotometrically by aluminium chloride colorimetric assay and 2-Diphenyl-1-picryl hydrazyl (DPPH) method respectively. The results showed that the choice of drying and extraction methods had a significant influence on total flavonoid and antioxidant content of C. nutans extract. The combination of drying C. nutans leaves using laboratory oven at 60°C and Soxhlet extraction obtained the highest amount of total flavonoid and antioxidant content at 24.53 ± 0.95 mg RU/g and 89.73 ± 4.39mg TE/ g respectively. The highest antioxidant activity for C. nutansfromair-dried samples and vacuum oven-dried samples were obtained through maceration extraction at 85.46 mg TE/ g and 83.96 mg TE/ g respectively.