Phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia

• Main Authors: Mohd Fadzelly Abu Bakar, Fifilyana Abdul Karim, Monica Suleiman, Azizul Isha, Asmah Rahmat
• Format: Article
• Language: English; English
• Published: Hindawi Publishing Corporation 2015
• Subjects: QK504-(638) Cryptogams
• Online Access: https://eprints.ums.edu.my/id/eprint/32224/3/Phytochemical%20constituents%2C%20antioxidant%20and%20antiproliferative%20properties%20of%20a%20liverwort%2C%20lepidozia%20borneensis%20Stephani%20from%20Mount%20Kinabalu%2C%20Sabah%2C%20Malaysia_ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/32224/5/Phytochemical%20constituents%2C%20antioxidant%20and%20antiproliferative%20properties%20of%20a%20liverwort%2C%20lepidozia%20borneensis%20Stephani%20from%20Mount%20Kinabalu%2C%20Sabah%2C%20Malaysia.pdf; https://eprints.ums.edu.my/id/eprint/32224/; https://downloads.hindawi.com/journals/ecam/2015/936215.pdf; http://dx.doi.org/10.1155/2015/936215
• Institution: Universiti Malaysia Sabah

The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents.