Preparation and characterisation of cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane

• Main Authors: Suryani Saallah, M. Nazli Naim, Mohd Noriznan Mokhtar, Noor Fitrah Abu Bakar, Masao Gen, Lenggoro, I. Wuled
• Format: Article
• Language: English; English
• Published: Society of Fiber Science and Technology 2021
• Subjects: TP1080-1185 Polymers and polymer manufacture
• Online Access: https://eprints.ums.edu.my/id/eprint/32594/1/Preparation%20and%20characterisation%20of%20cyclodextrin%20glucanotransferase%20enzyme%20immobilised%20in%20electrospun%20nanofibrous%20membrane%20_ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/32594/3/Preparation%20and%20characterisation%20of%20cyclodextrin%20glucanotransferase%20enzyme%20immobilised%20in%20electrospun%20nanofibrous%20membrane.pdf; https://eprints.ums.edu.my/id/eprint/32594/; https://www.jstage.jst.go.jp/article/fiberst/73/10/73_2017-0035/_pdf/-char/en; https://doi.org/10.2115/fiberst.2017-0035
• Institution: Universiti Malaysia Sabah

An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl alcohol (PVA) nanofiber (average diameter around 200 nm) membrane via co-electrospinning of the CGTase/PVA mixture followed with glutaraldehyde vapour phase cross-linking. Addition of enzyme with concentrati...