The circulating serotypes of dengue in Sabah, Malaysian Borneo

Background: Dengue is a mosquito borne arbovirus affecting humans and dengue infection has become a major public health problem in Asia Pacific countries. The virus is a positive sense, single-stranded enveloped RNA virus of the genus flavivirus. Malaysia is a dengue endemic country where all four d...

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Main Authors: Nadia Iryani Najri, Zulina Mazlan, Joel Judson Jaimin, Rashidah Mohammad, Tajul Ariffin Awang Mohd, Ahneez Abdul Hameed, Vijay Kumar Subbiah, Mohammad Zahirul Hoque
Format: Conference or Workshop Item
Language:English
English
Published: 2017
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Online Access:https://eprints.ums.edu.my/id/eprint/32641/1/The%20circulating%20serotypes%20of%20dengue%20in%20Sabah%2C%20Malaysian%20Borneo.pdf
https://eprints.ums.edu.my/id/eprint/32641/2/The%20circulating%20serotypes%20of%20dengue%20in%20Sabah%2C%20Malaysian%20Borneo1.pdf
https://eprints.ums.edu.my/id/eprint/32641/
https://atm.amegroups.com/article/view/16672/16914
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spelling my.ums.eprints.326412022-05-20T00:27:24Z https://eprints.ums.edu.my/id/eprint/32641/ The circulating serotypes of dengue in Sabah, Malaysian Borneo Nadia Iryani Najri Zulina Mazlan Joel Judson Jaimin Rashidah Mohammad Tajul Ariffin Awang Mohd Ahneez Abdul Hameed Vijay Kumar Subbiah Mohammad Zahirul Hoque QR355-502 Virology RA1-1270 Public aspects of medicine Background: Dengue is a mosquito borne arbovirus affecting humans and dengue infection has become a major public health problem in Asia Pacific countries. The virus is a positive sense, single-stranded enveloped RNA virus of the genus flavivirus. Malaysia is a dengue endemic country where all four dengue serotypes (DENV-1,-2,-3 and -4) have been reported but serotype data of Sabah is lacking. The aim of this study was to determine the circulating serotypes and other risk factors for dengue patients in Sabah. Methods: This was a cross sectional study, conducted during 2013–2015. A total 579 NS1- positive serum samples obtained from dengue patients were included from Public Health Laboratory of Sabah. All the samples had previously been tested for NS1 dengue using SD Bioline kit. Dengue viral RNA was extracted from NS1-positive serum using QIAmp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The NS1Ag positive samples were tested further by real time polymerase chain reaction (PCR) and reverse transcriptase PCR (RTPCR). Results: From 579 examined samples, 58% were from male patients and 42% were from females. Regarding circulating serotypes in the year 2013, DEN-4 was the predominant serotype at 84.54%, followed by DEN-1 (37.24%), DEN2 (18.12%) and DEN-3 (15.10%). In the year 2014, DEN1 was predominant (89.64%) followed by DEN-2 (31.22%), DEN-4 (10.75%) and DEN-3 (9.7%). Interestingly, in the year 2015, DEN-2 (45%) was predominant followed by DEN-1 (27%), DEN-4 (16%) and DEN-3 (12%). The number of patients with multiple serotype co-infections has seen increased in 2015. Conclusions: Our results show that the circulating serotypes is changing within different years. Therefore, early detection of circulating serotypes could be an important approach to prevent severe clinical outcomes during dengue outbreaks. It will be interesting to examine molecular characteristics and phylogenetic analysis of dengue in this geographical area. 2017 Conference or Workshop Item PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32641/1/The%20circulating%20serotypes%20of%20dengue%20in%20Sabah%2C%20Malaysian%20Borneo.pdf text en https://eprints.ums.edu.my/id/eprint/32641/2/The%20circulating%20serotypes%20of%20dengue%20in%20Sabah%2C%20Malaysian%20Borneo1.pdf Nadia Iryani Najri and Zulina Mazlan and Joel Judson Jaimin and Rashidah Mohammad and Tajul Ariffin Awang Mohd and Ahneez Abdul Hameed and Vijay Kumar Subbiah and Mohammad Zahirul Hoque (2017) The circulating serotypes of dengue in Sabah, Malaysian Borneo. In: The 12th Asia-Pacific Conference on Human Genetics, September 2017, Virtual. https://atm.amegroups.com/article/view/16672/16914
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR355-502 Virology
RA1-1270 Public aspects of medicine
spellingShingle QR355-502 Virology
RA1-1270 Public aspects of medicine
Nadia Iryani Najri
Zulina Mazlan
Joel Judson Jaimin
Rashidah Mohammad
Tajul Ariffin Awang Mohd
Ahneez Abdul Hameed
Vijay Kumar Subbiah
Mohammad Zahirul Hoque
The circulating serotypes of dengue in Sabah, Malaysian Borneo
description Background: Dengue is a mosquito borne arbovirus affecting humans and dengue infection has become a major public health problem in Asia Pacific countries. The virus is a positive sense, single-stranded enveloped RNA virus of the genus flavivirus. Malaysia is a dengue endemic country where all four dengue serotypes (DENV-1,-2,-3 and -4) have been reported but serotype data of Sabah is lacking. The aim of this study was to determine the circulating serotypes and other risk factors for dengue patients in Sabah. Methods: This was a cross sectional study, conducted during 2013–2015. A total 579 NS1- positive serum samples obtained from dengue patients were included from Public Health Laboratory of Sabah. All the samples had previously been tested for NS1 dengue using SD Bioline kit. Dengue viral RNA was extracted from NS1-positive serum using QIAmp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The NS1Ag positive samples were tested further by real time polymerase chain reaction (PCR) and reverse transcriptase PCR (RTPCR). Results: From 579 examined samples, 58% were from male patients and 42% were from females. Regarding circulating serotypes in the year 2013, DEN-4 was the predominant serotype at 84.54%, followed by DEN-1 (37.24%), DEN2 (18.12%) and DEN-3 (15.10%). In the year 2014, DEN1 was predominant (89.64%) followed by DEN-2 (31.22%), DEN-4 (10.75%) and DEN-3 (9.7%). Interestingly, in the year 2015, DEN-2 (45%) was predominant followed by DEN-1 (27%), DEN-4 (16%) and DEN-3 (12%). The number of patients with multiple serotype co-infections has seen increased in 2015. Conclusions: Our results show that the circulating serotypes is changing within different years. Therefore, early detection of circulating serotypes could be an important approach to prevent severe clinical outcomes during dengue outbreaks. It will be interesting to examine molecular characteristics and phylogenetic analysis of dengue in this geographical area.
format Conference or Workshop Item
author Nadia Iryani Najri
Zulina Mazlan
Joel Judson Jaimin
Rashidah Mohammad
Tajul Ariffin Awang Mohd
Ahneez Abdul Hameed
Vijay Kumar Subbiah
Mohammad Zahirul Hoque
author_facet Nadia Iryani Najri
Zulina Mazlan
Joel Judson Jaimin
Rashidah Mohammad
Tajul Ariffin Awang Mohd
Ahneez Abdul Hameed
Vijay Kumar Subbiah
Mohammad Zahirul Hoque
author_sort Nadia Iryani Najri
title The circulating serotypes of dengue in Sabah, Malaysian Borneo
title_short The circulating serotypes of dengue in Sabah, Malaysian Borneo
title_full The circulating serotypes of dengue in Sabah, Malaysian Borneo
title_fullStr The circulating serotypes of dengue in Sabah, Malaysian Borneo
title_full_unstemmed The circulating serotypes of dengue in Sabah, Malaysian Borneo
title_sort circulating serotypes of dengue in sabah, malaysian borneo
publishDate 2017
url https://eprints.ums.edu.my/id/eprint/32641/1/The%20circulating%20serotypes%20of%20dengue%20in%20Sabah%2C%20Malaysian%20Borneo.pdf
https://eprints.ums.edu.my/id/eprint/32641/2/The%20circulating%20serotypes%20of%20dengue%20in%20Sabah%2C%20Malaysian%20Borneo1.pdf
https://eprints.ums.edu.my/id/eprint/32641/
https://atm.amegroups.com/article/view/16672/16914
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