Isolation and culture of protoplasts from the medicinal plants Centella asiatica

• Main Authors: Zaleha A. Aziz, Davey, M. R., Lowe, K. C., Power, J. B.
• Format: Article
• Language: English; English
• Published: Sociedade Brasileira de Plantas Medicinais 2006
• Subjects: RS153-441 Materia medica; SB1-1110 Plant culture
• Online Access: https://eprints.ums.edu.my/id/eprint/33395/1/Isolation%20and%20culture%20of%20protoplasts%20from%20the%20medicinal%20plants%20Centella%20asiatica.pdf; https://eprints.ums.edu.my/id/eprint/33395/3/Isolation%20and%20culture%20of%20protoplasts%20from%20the%20medicinal%20plants%20Centella%20asiatica%20_ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/33395/; https://www1.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/8esp_105_109.pdf
• Institution: Universiti Malaysia Sabah

In the present investigation, protoplasts were isolated from cell suspensions initiated from leaf laminae and petioles using an enzyme mixture consisting of 1.5% (w/v) Cellulase R10, 1.0% (w/v) Macerozyme R10 and 0.5% (w/v) Driselase in CPW salts solution with 13% (w/v) mannitol as osmotic stabilize...