Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology

Understanding oil palma s cell wall lipid during pathogenic invasion, especially from Ganoderma fungus helps us to devise better strategies in managing Basal Stem Rot caused by the fungus effectively. However, as the lipids of planta s cell wall is a delicate subject, while acquiring them in ample a...

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Main Authors: Arnnyitte Alexander, Jedol Dayou, Coswald Stephen Sipaut @ Mohd Nasri, Khim-Phin Chong
Format: Article
Language:English
English
Published: ASM Science Journal 2021
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Online Access:https://eprints.ums.edu.my/id/eprint/36109/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/36109/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/36109/
https://doi.org/10.32802/asmscj.2021.659
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Institution: Universiti Malaysia Sabah
Language: English
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spelling my.ums.eprints.361092023-08-25T01:56:15Z https://eprints.ums.edu.my/id/eprint/36109/ Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology Arnnyitte Alexander Jedol Dayou Coswald Stephen Sipaut @ Mohd Nasri Khim-Phin Chong SB1-1110 Plant culture TK7800-8360 Electronics Understanding oil palma s cell wall lipid during pathogenic invasion, especially from Ganoderma fungus helps us to devise better strategies in managing Basal Stem Rot caused by the fungus effectively. However, as the lipids of planta s cell wall is a delicate subject, while acquiring them in ample amount with decent quality for research purpose is an excruciating work, therefore it is crucial to develops an optimised method for acquisition. In this study, the effects of solvent ratio, extraction time, extraction temperature and biomass to solvent ratio pertaining effective lipids extraction from oil palm roots cell wall were investigated, aided with Central Composite design (CCD) of Response Surface Methodology (RSM) analysis to obtain optimum oil palm cell wall lipid extraction conditions. Using the projection of RSM model and validation of empirical evaluation, the results revealed that the optimum cell wall lipids yield was obtained under the following extraction conditions: 10 mL of solvent mixture (chloroform: methanol (2:1, v/v)) per gram tissue for 120 min with gentle agitation at 30°C of extraction temperature. Ultimately, Fourier Transform Spectroscopy (FTIR) analysis was carried out to validate the successful and complete extraction were achieved using the optimised condition. ASM Science Journal 2021-07 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/36109/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/36109/2/FULL%20TEXT.pdf Arnnyitte Alexander and Jedol Dayou and Coswald Stephen Sipaut @ Mohd Nasri and Khim-Phin Chong (2021) Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology. ASM Science Journal, 16. pp. 1-10. ISSN 18236782 https://doi.org/10.32802/asmscj.2021.659
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic SB1-1110 Plant culture
TK7800-8360 Electronics
spellingShingle SB1-1110 Plant culture
TK7800-8360 Electronics
Arnnyitte Alexander
Jedol Dayou
Coswald Stephen Sipaut @ Mohd Nasri
Khim-Phin Chong
Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology
description Understanding oil palma s cell wall lipid during pathogenic invasion, especially from Ganoderma fungus helps us to devise better strategies in managing Basal Stem Rot caused by the fungus effectively. However, as the lipids of planta s cell wall is a delicate subject, while acquiring them in ample amount with decent quality for research purpose is an excruciating work, therefore it is crucial to develops an optimised method for acquisition. In this study, the effects of solvent ratio, extraction time, extraction temperature and biomass to solvent ratio pertaining effective lipids extraction from oil palm roots cell wall were investigated, aided with Central Composite design (CCD) of Response Surface Methodology (RSM) analysis to obtain optimum oil palm cell wall lipid extraction conditions. Using the projection of RSM model and validation of empirical evaluation, the results revealed that the optimum cell wall lipids yield was obtained under the following extraction conditions: 10 mL of solvent mixture (chloroform: methanol (2:1, v/v)) per gram tissue for 120 min with gentle agitation at 30°C of extraction temperature. Ultimately, Fourier Transform Spectroscopy (FTIR) analysis was carried out to validate the successful and complete extraction were achieved using the optimised condition.
format Article
author Arnnyitte Alexander
Jedol Dayou
Coswald Stephen Sipaut @ Mohd Nasri
Khim-Phin Chong
author_facet Arnnyitte Alexander
Jedol Dayou
Coswald Stephen Sipaut @ Mohd Nasri
Khim-Phin Chong
author_sort Arnnyitte Alexander
title Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology
title_short Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology
title_full Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology
title_fullStr Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology
title_full_unstemmed Optimisation of Cell Wall Lipids Extraction from Oil Palm using Mixture of Chloroform and Methanol via Response Surface Methodology
title_sort optimisation of cell wall lipids extraction from oil palm using mixture of chloroform and methanol via response surface methodology
publisher ASM Science Journal
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/36109/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/36109/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/36109/
https://doi.org/10.32802/asmscj.2021.659
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