In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo
Paphiopedilum lowii (Lindl.) Stein is alarmingly decreasing in numbers due to habitat destruction and over-collection. Propagation through seed is one effort to conserve the species. In vitro method offers better results for seed germination in orchids, but the success of germination is species-spec...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Society for Indonesian Biodiversity
2022
|
Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/36541/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/36541/2/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/36541/ https://doi.org/10.13057/biodiv/d231119 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Malaysia Sabah |
Language: | English English |
id |
my.ums.eprints.36541 |
---|---|
record_format |
eprints |
spelling |
my.ums.eprints.365412023-08-21T07:11:02Z https://eprints.ums.edu.my/id/eprint/36541/ In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo Vinishaa S. Ragu Roslin Ombokou Rimi Repin Duni Molidin Ramlan Miadin Zaleha Abdul Aziz QK495 Angiosperms Paphiopedilum lowii (Lindl.) Stein is alarmingly decreasing in numbers due to habitat destruction and over-collection. Propagation through seed is one effort to conserve the species. In vitro method offers better results for seed germination in orchids, but the success of germination is species-specific. Hence, this research aimed to evaluate different types of media [(½ strength Murashige & Skoog and Orchimax Medium (Duchefa Biochemie)], and to determine the effects of sucrose [0% 1%, 2% and 3% (w/v)], peptone [0%, 0.1% and 0.2% (w/v)], coconut water [0%, 10%, 20% and 30% (v/v)] and fertilizer [Orchid Focus-0%, 0.1% and 0.2% (v/v)] on seed germination. The protocorm size was also determined after 12 weeks of observation. Half-strength MS media consistently gave higher seed germination, ½ MS supplemented with 1% (w/v) sucrose, 0.1% (w/v) peptone, 0% (v/v) coconut water and 0.1 % (v/v) fertilizer resulted in the highest seed germination at 5.32% ± 5.9, 19.27% ± 9.34, 11.33% ± 3.80 and 19.31% ± 9.03 respectively. Despite being 12 weeks in culture, the sizes of the protocorms are small (0.222±0.089 mm diameter, 0.703±0.280 mm circumference, and 0.045±0.043 mm2 area); this requires future investigation. These findings can serve as base information for further enhancement of seed germination and development of propagation methods of P. lowii for use in a conservation program. © 2022, Society for Indonesian Biodiversity. All rights reserved. Society for Indonesian Biodiversity 2022-11-02 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/36541/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/36541/2/FULLTEXT.pdf Vinishaa S. Ragu and Roslin Ombokou and Rimi Repin and Duni Molidin and Ramlan Miadin and Zaleha Abdul Aziz (2022) In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo. Biodiversitas Journal of Biological Diversity, 23 (11). pp. 5687-5694. ISSN 1412-033X https://doi.org/10.13057/biodiv/d231119 |
institution |
Universiti Malaysia Sabah |
building |
UMS Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sabah |
content_source |
UMS Institutional Repository |
url_provider |
http://eprints.ums.edu.my/ |
language |
English English |
topic |
QK495 Angiosperms |
spellingShingle |
QK495 Angiosperms Vinishaa S. Ragu Roslin Ombokou Rimi Repin Duni Molidin Ramlan Miadin Zaleha Abdul Aziz In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo |
description |
Paphiopedilum lowii (Lindl.) Stein is alarmingly decreasing in numbers due to habitat destruction and over-collection. Propagation through seed is one effort to conserve the species. In vitro method offers better results for seed germination in orchids, but the success of germination is species-specific. Hence, this research aimed to evaluate different types of media [(½ strength Murashige & Skoog and Orchimax Medium (Duchefa Biochemie)], and to determine the effects of sucrose [0% 1%, 2% and 3% (w/v)], peptone [0%, 0.1% and 0.2% (w/v)], coconut water [0%, 10%, 20% and 30% (v/v)] and fertilizer [Orchid Focus-0%, 0.1% and 0.2% (v/v)] on seed germination. The protocorm size was also determined after 12 weeks of observation. Half-strength MS media consistently gave higher seed germination, ½ MS supplemented with 1% (w/v) sucrose, 0.1% (w/v) peptone, 0% (v/v) coconut water and 0.1 % (v/v) fertilizer resulted in the highest seed germination at 5.32% ± 5.9, 19.27% ± 9.34, 11.33% ± 3.80 and 19.31% ± 9.03 respectively. Despite being 12 weeks in culture, the sizes of the protocorms are small (0.222±0.089 mm diameter, 0.703±0.280 mm circumference, and 0.045±0.043 mm2 area); this requires future investigation. These findings can serve as base information for further enhancement of seed germination and development of propagation methods of P. lowii for use in a conservation program. © 2022, Society for Indonesian Biodiversity. All rights reserved. |
format |
Article |
author |
Vinishaa S. Ragu Roslin Ombokou Rimi Repin Duni Molidin Ramlan Miadin Zaleha Abdul Aziz |
author_facet |
Vinishaa S. Ragu Roslin Ombokou Rimi Repin Duni Molidin Ramlan Miadin Zaleha Abdul Aziz |
author_sort |
Vinishaa S. Ragu |
title |
In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo |
title_short |
In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo |
title_full |
In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo |
title_fullStr |
In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo |
title_full_unstemmed |
In vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in North Borneo |
title_sort |
in vitro seed germination of paphiopedilum lowii, an endangered slipper orchid in north borneo |
publisher |
Society for Indonesian Biodiversity |
publishDate |
2022 |
url |
https://eprints.ums.edu.my/id/eprint/36541/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/36541/2/FULLTEXT.pdf https://eprints.ums.edu.my/id/eprint/36541/ https://doi.org/10.13057/biodiv/d231119 |
_version_ |
1775623867596800000 |