Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids

Context: Bauhinia purpurea L. (Fabaceae) is used in the Ayurvedic system to treat various oxidativerelated ailments (e.g., wounds, ulcers etc.). Therefore, it is believed that the plant also has the potential to alleviate oxidative-related liver damage. Objective: This study elucidates the hepatopro...

Full description

Saved in:
Bibliographic Details
Main Authors: Zainul Amiruddin Zakaria, Adibah Sahmat, Azfar Hizami Azm, Amal Syahirah Nur Zainol, Maizatul Hasyima Omar, Tavamani Balan, Arifah Abdul Kadir, Syahriel Abdullah, Roro Azizah, Lilis Sulistyorini
Format: Article
Language:English
English
Published: Informa Healthcare 2023
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/38244/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/38244/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/38244/
https://doi.org/10.1080/13880209.2023.2241510
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Malaysia Sabah
Language: English
English
id my.ums.eprints.38244
record_format eprints
spelling my.ums.eprints.382442024-02-15T02:37:17Z https://eprints.ums.edu.my/id/eprint/38244/ Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids Zainul Amiruddin Zakaria Adibah Sahmat Azfar Hizami Azm Amal Syahirah Nur Zainol Maizatul Hasyima Omar Tavamani Balan Arifah Abdul Kadir Syahriel Abdullah Roro Azizah Lilis Sulistyorini GT2400-3390.5 Customs relative to private life Including children, marriage, eating and drinking, funeral customs, etc. RA1190-1270 Toxicology. Poisons Context: Bauhinia purpurea L. (Fabaceae) is used in the Ayurvedic system to treat various oxidativerelated ailments (e.g., wounds, ulcers etc.). Therefore, it is believed that the plant also has the potential to alleviate oxidative-related liver damage. Objective: This study elucidates the hepatoprotective activity of chloroform extract of B. purpurea leaves (CEBP) in paracetamol (PCM)-induced liver injury (PILI) rats. Materials and methods: Male Sprague-Dawley rats (n ¼ 6) were pre-treated once daily (p.o.) with CEBP (50–500 mg/kg) for seven consecutive days before being administered (p.o.) a hepatotoxic agent, 3 g/kg PCM. Liver enzyme levels were determined from the collected blood, while the collected liver was used to determine the activity of endogenous antioxidant enzymes and for histopathological examination. CEBP was also subjected to radical scavenging assays and phytochemical analysis. Results: CEBP significantly (p < 0.05) reversed the toxic effect of PCM by increasing the serum levels of AST and ALT, and the activity of endogenous catalase (CAT) and superoxide dismutase (SOD) while reducing the liver weight/body weight (LW/BW) ratio. Other than low TPC value and radical scavenging activity, CEBP had a high antioxidant capacity when evaluated using the oxygen radical absorbance capacity (ORAC) assay. UHPLC-ESI-MS analysis of CEBP showed the presence of flavonoids. Discussion and conclusions: CEBP exerts its hepatoprotective activity through a non-free radical scavenging pathway that involves activation of the endogenous enzymatic antioxidant defense system. Further study is needed to identify the responsible bioactive compounds before the plant can be developed as a future alternative hepatoprotective medicament for clinical use. Informa Healthcare 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38244/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38244/2/FULL%20TEXT.pdf Zainul Amiruddin Zakaria and Adibah Sahmat and Azfar Hizami Azm and Amal Syahirah Nur Zainol and Maizatul Hasyima Omar and Tavamani Balan and Arifah Abdul Kadir and Syahriel Abdullah and Roro Azizah and Lilis Sulistyorini (2023) Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids. Pharmaceutical Biology, 61 (1). pp. 1-10. ISSN 13880209 https://doi.org/10.1080/13880209.2023.2241510
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic GT2400-3390.5 Customs relative to private life Including children, marriage, eating and drinking, funeral customs, etc.
RA1190-1270 Toxicology. Poisons
spellingShingle GT2400-3390.5 Customs relative to private life Including children, marriage, eating and drinking, funeral customs, etc.
RA1190-1270 Toxicology. Poisons
Zainul Amiruddin Zakaria
Adibah Sahmat
Azfar Hizami Azm
Amal Syahirah Nur Zainol
Maizatul Hasyima Omar
Tavamani Balan
Arifah Abdul Kadir
Syahriel Abdullah
Roro Azizah
Lilis Sulistyorini
Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
description Context: Bauhinia purpurea L. (Fabaceae) is used in the Ayurvedic system to treat various oxidativerelated ailments (e.g., wounds, ulcers etc.). Therefore, it is believed that the plant also has the potential to alleviate oxidative-related liver damage. Objective: This study elucidates the hepatoprotective activity of chloroform extract of B. purpurea leaves (CEBP) in paracetamol (PCM)-induced liver injury (PILI) rats. Materials and methods: Male Sprague-Dawley rats (n ¼ 6) were pre-treated once daily (p.o.) with CEBP (50–500 mg/kg) for seven consecutive days before being administered (p.o.) a hepatotoxic agent, 3 g/kg PCM. Liver enzyme levels were determined from the collected blood, while the collected liver was used to determine the activity of endogenous antioxidant enzymes and for histopathological examination. CEBP was also subjected to radical scavenging assays and phytochemical analysis. Results: CEBP significantly (p < 0.05) reversed the toxic effect of PCM by increasing the serum levels of AST and ALT, and the activity of endogenous catalase (CAT) and superoxide dismutase (SOD) while reducing the liver weight/body weight (LW/BW) ratio. Other than low TPC value and radical scavenging activity, CEBP had a high antioxidant capacity when evaluated using the oxygen radical absorbance capacity (ORAC) assay. UHPLC-ESI-MS analysis of CEBP showed the presence of flavonoids. Discussion and conclusions: CEBP exerts its hepatoprotective activity through a non-free radical scavenging pathway that involves activation of the endogenous enzymatic antioxidant defense system. Further study is needed to identify the responsible bioactive compounds before the plant can be developed as a future alternative hepatoprotective medicament for clinical use.
format Article
author Zainul Amiruddin Zakaria
Adibah Sahmat
Azfar Hizami Azm
Amal Syahirah Nur Zainol
Maizatul Hasyima Omar
Tavamani Balan
Arifah Abdul Kadir
Syahriel Abdullah
Roro Azizah
Lilis Sulistyorini
author_facet Zainul Amiruddin Zakaria
Adibah Sahmat
Azfar Hizami Azm
Amal Syahirah Nur Zainol
Maizatul Hasyima Omar
Tavamani Balan
Arifah Abdul Kadir
Syahriel Abdullah
Roro Azizah
Lilis Sulistyorini
author_sort Zainul Amiruddin Zakaria
title Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
title_short Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
title_full Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
title_fullStr Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
title_full_unstemmed Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
title_sort liver protective effect of chloroform extract of bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids
publisher Informa Healthcare
publishDate 2023
url https://eprints.ums.edu.my/id/eprint/38244/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/38244/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/38244/
https://doi.org/10.1080/13880209.2023.2241510
_version_ 1792152916125024256