Cryopreservation of the North Borneo Phalaenopsis gigantea J.J.Sm. Using a Vitrification Approach

• Main Authors: Zaleha A. Aziz, Mariani Ahmad, Low, Wan Chin Chin, Rimi Repin, Simun Limbawang, Ramlan Maidin, Rosmah Murdad
• Format: Article
• Language: English; English
• Published: Institute for Tropical Biology and Conservation 2023
• Subjects: QH301-705.5 Biology (General); SB403-450.87 Flowers and flower culture. Ornamental plants
• Online Access: https://eprints.ums.edu.my/id/eprint/39274/1/ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/39274/2/FULL%20TEXT.pdf; https://eprints.ums.edu.my/id/eprint/39274/; https://doi.org/10.51200/jtbc.v20i.4651
• Institution: Universiti Malaysia Sabah

Phalaenopsis gigantea is an endangered orchid found in Borneo and Kalimantan. A cryopreservation protocol using vitrification was developed using seeds as explants. Seeds from green unburst capsules were precultured on New Dogashima Medium supplemented with 0-0.5 M sucrose prior to treatment with lo...