Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq
Published in Middle-East Journal of Scientific Research 14 (2): 155-160, 2013. Full text also available at http://www.idosi.org/mejsr/mejsr14%282%2913/2.pdf
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2014
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my.unikl.ir-77522014-09-11T04:15:39Z Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq S. Al-Jassabi Ali Saad T.R. Satyakeerthy M.S. Abdullah (UniKL RCMP) Polyphenol Oxidase Zyzyphus Spina Christi Catechol Published in Middle-East Journal of Scientific Research 14 (2): 155-160, 2013. Full text also available at http://www.idosi.org/mejsr/mejsr14%282%2913/2.pdf Polyphenol oxidase (PPO) from Zyzyphus spina christi found in central part of Iraq was extracted and purified by (NH ) SO precipitation, ion-exchange chromatography and gel filtration chromatography. 42 4 The biochemical characteristics reveal that the PPO from Zyzyphus spina christi has higher affinity towards catechol ( K = 11.4mM and V = 16,400 U/ml min ) at an optimum pH of 5.8. The enzyme had an optimum M max 1 temperature of 37°C and was relatively stable up to 50°C for a period of 60 minutes with almost 80% activity remaining. Among the various PPO inhibitors tested, the most effective inhibitor for the enzyme with 10mM catechol as substrate was ascorbic acid 2014-09-11T04:15:39Z 2014-09-11T04:15:39Z 2013 Middle-East Journal of Scientific Research DOI: 10.5829/idosi.mejsr.2013.14.2.7373 http://localhost/xmlui/handle/123456789/7752 IDOSI Publications |
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| topic |
Polyphenol Oxidase Zyzyphus Spina Christi Catechol |
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Polyphenol Oxidase Zyzyphus Spina Christi Catechol S. Al-Jassabi Ali Saad T.R. Satyakeerthy M.S. Abdullah (UniKL RCMP) Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
| description |
Published in Middle-East Journal of Scientific Research 14 (2): 155-160, 2013. Full text also available at http://www.idosi.org/mejsr/mejsr14%282%2913/2.pdf |
| format |
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| author |
S. Al-Jassabi Ali Saad T.R. Satyakeerthy M.S. Abdullah (UniKL RCMP) |
| author_facet |
S. Al-Jassabi Ali Saad T.R. Satyakeerthy M.S. Abdullah (UniKL RCMP) |
| author_sort |
S. Al-Jassabi |
| title |
Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
| title_short |
Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
| title_full |
Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
| title_fullStr |
Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
| title_full_unstemmed |
Characterization of Polyphenol Oxidase from Zyzyphus spina-christi from Iraq |
| title_sort |
characterization of polyphenol oxidase from zyzyphus spina-christi from iraq |
| publisher |
IDOSI Publications |
| publishDate |
2014 |
| url |
http://localhost/xmlui/handle/123456789/7752 |
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1644484928667123712 |