Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger

Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger

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Bibliographic Details
Main Author: Raja Ahmad Izzat, Raja Zazali
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Pectinase
Rice bran
Aspergillus niger
Solid state fermentation
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/16527
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Institution: Universiti Malaysia Perlis
Language: English
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spelling my.unimap-165272011-12-01T04:44:20Z Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger Raja Ahmad Izzat, Raja Zazali Mohamad Fahrurrazi Tompang Pectinase Rice bran Aspergillus niger Solid state fermentation Access is limited to UniMAP community. Pectinase is an important hydrolytic enzyme playing a vital role in hydrolyzing pectin molecules that contribute to the degradation of pectin by various mechanisms. Rice bran is an agro-waste from the process of rice milling to produce white rice from brown rice. The microorganism used is Aspergillus niger strain ATCC 16404. The aim of this research is to optimize pectinase production from rice bran based on media composition which is the carbon and nitrogen sources. In addition, it is desired to study the effect of interaction between the parameters, sucrose (1.0-3.0 % w/w) as carbon source and ammonium sulphate (0.3-0.7 % w/w) as nitrogen source. The research was done by using Central Composite Design (CCD) under Response Surface Methodology (RSM). Based on the screening of fermentation period, three days of incubation period was considered as the optimum time for solid state fermentation. In the optimization process, the design contains a total of 11 experimental runs involving replications of central points and organized in a randomized factorial design. From the analysis of variance (ANOVA) using quadratic polynomial equation, maximum pectinase activity of 9.552 U/g was obtained using 2 % (w/w) sucrose and 0.5 % (w/w) ammonium sulphate. 2011-12-01T04:44:20Z 2011-12-01T04:44:20Z 2011-05 Learning Object http://hdl.handle.net/123456789/16527 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Pectinase
Rice bran
Aspergillus niger
Solid state fermentation
spellingShingle Pectinase
Rice bran
Aspergillus niger
Solid state fermentation
Raja Ahmad Izzat, Raja Zazali
Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Raja Ahmad Izzat, Raja Zazali
format Learning Object
author Raja Ahmad Izzat, Raja Zazali
author_sort Raja Ahmad Izzat, Raja Zazali
title Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
title_short Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
title_full Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
title_fullStr Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
title_full_unstemmed Media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
title_sort media optimization for pectinase production from rice bran by solid state bioconversion using aspergillus niger
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/16527
_version_ 1643791062622273536

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