Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
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Universiti Malaysia Perlis
2011
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my.unimap-170732011-12-08T07:49:17Z Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste Nurul Afidah, Umoruddin Syazni Zainul Kamal Cellulases Aspergillus niger Hydrolytic enzymes Cassava waste Access is limited to UniMAP community. Cellulases are a group of hydrolytic enzymes and are capable of degrading lignocellulosic materials contain carbohydrate polymers such as cellulose, hemicelulose and starch. Cellulases have wide range of applications. In this study, cellulase production was carried out in solid-state fermentation using cassava waste as a substrate by using Aspergillus niger. Different fermentation conditions were examined to assess their effect in optimizing enzyme production. The effects of various process variables were studied for optimum production of cellulase enzyme using cassava waste including moisture content (%), fermentation time (day) and inoculums size (ml). Fermentation of A.niger with cassava waste substrate will produce cellulases. Cellulases enzyme will degrade cellulose to the smaller glucose units. The production of cellulase was determined based on glucose concentration. Full factorial composite experimental design and central composite design (CCD) were used in the design of experiments and analysis of results. The result indicated that the optimal condition for fermentation of 5 g cassava waste substrate by A.niger was obtained at 100 % of moisture content, 7.95 days of fermentation and 3.78 ml of inoculums size. The coefficient of determination obtained was 0.9125 shows the model adequacy was very satisfactory. 2011-12-08T07:49:16Z 2011-12-08T07:49:16Z 2011-04 Learning Object http://hdl.handle.net/123456789/17073 en Universiti Malaysia Perlis School of Bioprocess Engineering |
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Cellulases Aspergillus niger Hydrolytic enzymes Cassava waste |
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Cellulases Aspergillus niger Hydrolytic enzymes Cassava waste Nurul Afidah, Umoruddin Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste |
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Syazni Zainul Kamal |
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Syazni Zainul Kamal Nurul Afidah, Umoruddin |
format |
Learning Object |
author |
Nurul Afidah, Umoruddin |
author_sort |
Nurul Afidah, Umoruddin |
title |
Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste |
title_short |
Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste |
title_full |
Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste |
title_fullStr |
Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste |
title_full_unstemmed |
Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste |
title_sort |
physical parameter optimization of cellulase production by aspergillus niger on solid-state fermentation of cassava waste |
publisher |
Universiti Malaysia Perlis |
publishDate |
2011 |
url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/17073 |
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1643791032803917824 |