Development of high voltage pulse inducement method for biological cell
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Institute of Electrical and Electronics Engineers (IEEE)
2012
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my.unimap-213852012-10-17T09:15:19Z Development of high voltage pulse inducement method for biological cell Mohamad Nazib, Adon Mohd Noh, Dalimin, Prof. Dato' Dr. Muhammad Mahadi, Abdul Jamil, Dr. Norazan, Mohd Kassim nazib@uthm.edu.my Electroporation (EP) High voltage pulse Real time monitoring Electromagnetic field Link to publisher's homepage at http://ieeexplore.ieee.org/ Electroporation (EP) system is a process of controlling cell functions by using electromagnetic fields (EMF) to create pores through a cellular membrane that causes cell lysis and apoptosis. In this paper we present an experimental setup for fundamental studies on cell EP. An adjustable high voltage pulse generator (3kV/10μs – 600μs pulse length) system were connected to the EP chamber which subsequently allow real time observation of membrane permeability changes and cellular physiology. In order to initiate higher cell viability rate, high transfection efficiency, lower sample contamination and smaller Joule heating the modification of EP chamber need to be implemented. Following that, HeLa cell culture has been projected as cell that will be used in this study. Finally, some suggestions are proposed for the future studies. 2012-10-17T09:15:19Z 2012-10-17T09:15:19Z 2012-02-27 Working Paper p. 501-503 978-145771989-9 http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6179068 http://hdl.handle.net/123456789/21385 en Proceedings of the International Conference on Biomedical Engineering (ICoBE 2012) Institute of Electrical and Electronics Engineers (IEEE) |
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| language |
English |
| topic |
Electroporation (EP) High voltage pulse Real time monitoring Electromagnetic field |
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Electroporation (EP) High voltage pulse Real time monitoring Electromagnetic field Mohamad Nazib, Adon Mohd Noh, Dalimin, Prof. Dato' Dr. Muhammad Mahadi, Abdul Jamil, Dr. Norazan, Mohd Kassim Development of high voltage pulse inducement method for biological cell |
| description |
Link to publisher's homepage at http://ieeexplore.ieee.org/ |
| author2 |
nazib@uthm.edu.my |
| author_facet |
nazib@uthm.edu.my Mohamad Nazib, Adon Mohd Noh, Dalimin, Prof. Dato' Dr. Muhammad Mahadi, Abdul Jamil, Dr. Norazan, Mohd Kassim |
| format |
Working Paper |
| author |
Mohamad Nazib, Adon Mohd Noh, Dalimin, Prof. Dato' Dr. Muhammad Mahadi, Abdul Jamil, Dr. Norazan, Mohd Kassim |
| author_sort |
Mohamad Nazib, Adon |
| title |
Development of high voltage pulse inducement method for biological cell |
| title_short |
Development of high voltage pulse inducement method for biological cell |
| title_full |
Development of high voltage pulse inducement method for biological cell |
| title_fullStr |
Development of high voltage pulse inducement method for biological cell |
| title_full_unstemmed |
Development of high voltage pulse inducement method for biological cell |
| title_sort |
development of high voltage pulse inducement method for biological cell |
| publisher |
Institute of Electrical and Electronics Engineers (IEEE) |
| publishDate |
2012 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/21385 |
| _version_ |
1643793389694484480 |