Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction
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2013
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my.unimap-235522013-02-14T08:01:11Z Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Thikra, S. Dhahi Mustafa, Shuhaimi, Assoc. Prof. Madya Dr. Yaakob, Che Man, Prof. Dato' Dr. Md Abdul Latif, Dr. Burger formulation Double quenched TaqMan probe Halal and Kosher foods PLS model Link to publisher's homepage at http://link.springer.com/ A TaqMan probe real-time polymerase chain reaction assay was developed for the determination of pork adulteration in commercial burgers. The assay combined porcine-specific primers and TaqMan probe for the selective amplification and detection of a 109-bp fragment of swine cytochrome b (cytb) gene. Specificity test with 10 ng DNA of 11 different meat-providing animal and fish species yielded a quantification cycle (Cq) of 15. 5 ± 0. 20 for the pork and negative results for the others in a 40-cycle reaction with a change of analysts and sources. Analysis of beef burger formulations with spiked pork showed the assay can determine 100-0. 01% contaminated pork with a PCR efficiency (E) of 93. 8% and a correlation coefficient (R 2) of 0. 991. A plot of actual value against real-time PCR-predicted value also yielded a good linear regression, R 2 0. 998, and small root mean square error of calibration, RMSEC 0. 42. A strong correlation was found between the partial least square (PLS)-predicted values and real-time PCR-determined values. The accuracy of the method was ≥90% in all determinations of the standard set. Residual analysis also revealed a high precision in all determinations. Finally, a random analysis of 10 ng DNA of commercial burgers from pork, beef, chicken, mutton, and chevon yielded a Cq of 15. 56 ± 0. 22 to 16. 24 ± 0. 35 from pork burgers, and negative results from the others, showing the suitability of the assay to determine pork in commercial burgers with a high accuracy and precision. 2013-02-14T08:01:11Z 2013-02-14T08:01:11Z 2012-08 Article Food Analytical Methods, vol. 5 (4), 2012, pages 784-794 1936-9751 (Print) 1936-976X (Online) http://link.springer.com/article/10.1007%2Fs12161-011-9311-4 http://hdl.handle.net/123456789/23552 en Springer-Verlag |
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Burger formulation Double quenched TaqMan probe Halal and Kosher foods PLS model |
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Burger formulation Double quenched TaqMan probe Halal and Kosher foods PLS model Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Thikra, S. Dhahi Mustafa, Shuhaimi, Assoc. Prof. Madya Dr. Yaakob, Che Man, Prof. Dato' Dr. Md Abdul Latif, Dr. Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction |
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Link to publisher's homepage at http://link.springer.com/ |
| format |
Article |
| author |
Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Thikra, S. Dhahi Mustafa, Shuhaimi, Assoc. Prof. Madya Dr. Yaakob, Che Man, Prof. Dato' Dr. Md Abdul Latif, Dr. |
| author_facet |
Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Thikra, S. Dhahi Mustafa, Shuhaimi, Assoc. Prof. Madya Dr. Yaakob, Che Man, Prof. Dato' Dr. Md Abdul Latif, Dr. |
| author_sort |
Md. Eaqub, Ali |
| title |
Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction |
| title_short |
Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction |
| title_full |
Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction |
| title_fullStr |
Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction |
| title_full_unstemmed |
Analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome B gene by TaqMan probe real-time polymerase chain reaction |
| title_sort |
analysis of pork adulteration in commercial burgers targeting porcine-specific mitochondrial cytochrome b gene by taqman probe real-time polymerase chain reaction |
| publisher |
Springer-Verlag |
| publishDate |
2013 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/23552 |
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