Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
Organized by Institute of Research, Development and Commercialisation (IRDC), UiTM, 3rd - 5th July 2007 at Sunway Lagoon Resort Hotel, Selangor.
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Universiti Teknologi MARA (UiTM)
2008
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my.unimap-26392009-12-31T04:09:26Z Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers Mohd Azlan, Mohd Ishak Zarina, Zakaria Abd Hamid, Khalid Suhana, Zakaria Essences and essential oils Plants -- Analysis Citrus grandis Gas chromatography Fragrance research Organized by Institute of Research, Development and Commercialisation (IRDC), UiTM, 3rd - 5th July 2007 at Sunway Lagoon Resort Hotel, Selangor. Citrus grandis or pomelo flowers possess a strong floral-, jasmine- and orange-like fragrance. In this study, various sampling techniques such as hydrodistillation, Soxhlet extraction and Solid Phase Micro extraction (SPME) were used to compare the extraction capability of the fragrance from pomelo flowers. Gas Chromatograph - Mass Spectrometry (GCMS) was then used to identify the extracted components. Results thus far showed that, limonene, linalool, ocimene, naphthalene and caryophyllene were the major compounds detected. In the Soxhlet extraction method, limonene was a major compound detected on extracts of blossoms and buds with percent concentrations of 4.4 and 9.3%, respectively. On the contrary, analysis on extracts of both flower components via the hydrodistillation method was identified linalool as the major compound with percent concentrations of 8.3 and 2.7%, respectively. In the SPME method, however, the detection of limonene in both flower components was found to be the most efficient with the peak area of ca. 13 and 62%, respectively. In this method, three different types of coating fibres were evaluated and 100 µm polydimethylsiloxane coated fibre showed a high efficiency detection of fragrance compounds from pomelo flowers. Analysis of buds showed that, limonene attained the highest relative abundance followed by ocimene, caryophyllene and naphthalene. In tissue culture procedure, all six different parts of cultured explants namely petal, sepal, style, ovary, pistil and cup base showed a capability to form callus as early as day 5. Style and pistil were the best explants to form callus on Murashige and Skoog media which supplemented with 0.05 mg/l and 0.01 mg/l BAP, respectively. 2008-10-29T07:38:17Z 2008-10-29T07:38:17Z 2007-07-05 Working Paper http://hdl.handle.net/123456789/2639 en Proceedings of the Conference on Scientific & Social Research 2006/2007 (CSSR 06/07) Universiti Teknologi MARA (UiTM) |
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Universiti Malaysia Perlis |
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English |
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Essences and essential oils Plants -- Analysis Citrus grandis Gas chromatography Fragrance research |
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Essences and essential oils Plants -- Analysis Citrus grandis Gas chromatography Fragrance research Mohd Azlan, Mohd Ishak Zarina, Zakaria Abd Hamid, Khalid Suhana, Zakaria Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers |
| description |
Organized by Institute of Research, Development and Commercialisation (IRDC), UiTM, 3rd - 5th July 2007 at Sunway Lagoon Resort Hotel, Selangor. |
| format |
Working Paper |
| author |
Mohd Azlan, Mohd Ishak Zarina, Zakaria Abd Hamid, Khalid Suhana, Zakaria |
| author_facet |
Mohd Azlan, Mohd Ishak Zarina, Zakaria Abd Hamid, Khalid Suhana, Zakaria |
| author_sort |
Mohd Azlan, Mohd Ishak |
| title |
Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers |
| title_short |
Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers |
| title_full |
Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers |
| title_fullStr |
Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers |
| title_full_unstemmed |
Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers |
| title_sort |
characterisation of fragrance major compouds in intact plant and in vitro cultures of citrus grandis (osbeck) flowers |
| publisher |
Universiti Teknologi MARA (UiTM) |
| publishDate |
2008 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/2639 |
| _version_ |
1643787705769787392 |