Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse

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Main Author:	Muhammad Lokman, Muhammad Ramdan
Other Authors:	Syazni Zainul Kamal
Format:	Learning Object
Language:	English
Published:	Universiti Malaysia Perlis (UniMAP) 2013
Subjects:	Cellulose Aspergillus niger Sugarcane bagasse Cellulase enzyme
Online Access:	http://dspace.unimap.edu.my/xmlui/handle/123456789/27664
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Institution:	Universiti Malaysia Perlis
Language:	English

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spelling	my.unimap-276642013-08-26T07:47:30Z Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse Muhammad Lokman, Muhammad Ramdan Syazni Zainul Kamal Cellulose Aspergillus niger Sugarcane bagasse Cellulase enzyme Access is limited to UniMAP community. Cellulase can be produced using fungi such as Aspergillus niger on solid state fermentation (SSF). But, the problem is the cost of producing the enzyme is expensive. The growing approaches to reduce the cost of cellulases production can be achieved via using of cheap, renewable agriculture wastes which consider an excellent carbon source for microbial enzymes production and also looking for microorganism having the ability to hyper-production of cellulases. Therefore, considering the advantages of SSF, the optimal chemical condition for the production of cellulase enzyme by Apergillus niger from solid-state fermentation of sugarcane bagasse is investigated. Three parameters was chosen to be screened and optimized using Design Expert Software (DOE). The parameters are amount of substrate (C-source), concentration of yeast extract (N-source) and concentration of Potassium di-Hydrogen Phosphate (KH2PO4) (Phosphate source). After screening, the parameter for N-source was not significant to the model. Thus, parameters C-source and Phosphate source was optimize. The optimum point predictions using DOE are 7.20 g for the amount of substrate and 3.6 g/L of KH2PO4 which resulted in enzyme activity of 0.356562 IU/mL. 2013-08-26T07:47:30Z 2013-08-26T07:47:30Z 2012-06 Learning Object http://hdl.handle.net/123456789/27664 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
building	UniMAP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Perlis
content_source	UniMAP Library Digital Repository
url_provider	http://dspace.unimap.edu.my/
language	English
topic	Cellulose Aspergillus niger Sugarcane bagasse Cellulase enzyme
spellingShingle	Cellulose Aspergillus niger Sugarcane bagasse Cellulase enzyme Muhammad Lokman, Muhammad Ramdan Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
description	Access is limited to UniMAP community.
author2	Syazni Zainul Kamal
author_facet	Syazni Zainul Kamal Muhammad Lokman, Muhammad Ramdan
format	Learning Object
author	Muhammad Lokman, Muhammad Ramdan
author_sort	Muhammad Lokman, Muhammad Ramdan
title	Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_short	Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_full	Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_fullStr	Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_full_unstemmed	Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_sort	chemical parameter optimization of cellulase production by aspergillus niger on solid state fermentation of sugarcane bagasse
publisher	Universiti Malaysia Perlis (UniMAP)
publishDate	2013
url	http://dspace.unimap.edu.my/xmlui/handle/123456789/27664
_version_	1643795385299238912

Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse

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