Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics

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Main Author:	Chen, Hui Wen
Other Authors:	Zulkarnain Mohd Idris
Format:	Learning Object
Language:	English
Published:	Universiti Malaysia Perlis (UniMAP) 2013
Subjects:	Antioxidant Phanerochaete chrysosporium Bioactive phenolics White rot fungus Basidiomycetes
Online Access:	http://dspace.unimap.edu.my/xmlui/handle/123456789/27734
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Institution:	Universiti Malaysia Perlis
Language:	English

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spelling	my.unimap-277342013-08-27T08:35:11Z Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics Chen, Hui Wen Zulkarnain Mohd Idris Antioxidant Phanerochaete chrysosporium Bioactive phenolics White rot fungus Basidiomycetes Access is limited to UniMAP community. Phanerochaete chrysosporium or known as white rot fungus has the major ability in lignin-degradable. However, antioxidant activities of the extracts from Phanerochaete chrysosporium biomass are poorly reported. In this study, the extraction conditions for antioxidant activity of Phanerochaete chrysosporium biomass extracts were determined by using Plackett-Burman experimental design. The extraction conditions studied were solid-solvent ratio, solvent concentration, agitation speed, extraction pH, temperature, and time. Positive effect variables such as solid-solvent ratio, pH, and time were found to be 24.19, 23.57, and 2.82 respectively. On contrary, agitation speed, solvent concentration and temperature had negatively effects on antioxidant activity at -0.45, -13.73, and -18.42 respectively. Folin-Ciocalteu reagents were used to determine the total phenolic compounds while 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay was used to analyze the antioxidant activity. From the results, there were no correlation between total phenolic compounds and antioxidant activity. But Phanerochaete chrysosporium biomass extract still demonstrated antioxidant activity where the most potent radical scavenging activity showing 84.21±0.01% compares with butylated hydroxytoluene (BHT) that showed 84.5 ±0.01%. 2013-08-27T08:35:11Z 2013-08-27T08:35:11Z 2012-06 Learning Object http://hdl.handle.net/123456789/27734 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
building	UniMAP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Perlis
content_source	UniMAP Library Digital Repository
url_provider	http://dspace.unimap.edu.my/
language	English
topic	Antioxidant Phanerochaete chrysosporium Bioactive phenolics White rot fungus Basidiomycetes
spellingShingle	Antioxidant Phanerochaete chrysosporium Bioactive phenolics White rot fungus Basidiomycetes Chen, Hui Wen Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
description	Access is limited to UniMAP community.
author2	Zulkarnain Mohd Idris
author_facet	Zulkarnain Mohd Idris Chen, Hui Wen
format	Learning Object
author	Chen, Hui Wen
author_sort	Chen, Hui Wen
title	Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_short	Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_full	Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_fullStr	Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_full_unstemmed	Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_sort	effects of extraction conditions on antioxidant activity of phanerochaete chrysosporium bioactive phenolics
publisher	Universiti Malaysia Perlis (UniMAP)
publishDate	2013
url	http://dspace.unimap.edu.my/xmlui/handle/123456789/27734
_version_	1643795390877663232

Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics

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