Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
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my.unimap-327782014-03-15T07:42:49Z Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural Hasunuma, Tomohisa Ku Syahidah, Ku Ismail Nambu, Yumiko Kondo, Akihiko hasunuma@port.kobe-u.ac.jp kusyahidah@unimap.edu.my nambu@people.kobe-u.ac.jp akondo@kobe-u.ac.jp Furfural TAL1 ADH1 Overexpression Hemicellulosic hydrolysate Xylose Saccharomyces cerevisiae Bioethanol Link to publisher's homepage at http://www.elsevier.com Lignocellulosic biomass dedicated to bioethanol production usually contains pentoses and inhibitory compounds such as furfural that are not well tolerated by Saccharomyces cerevisiae. Thus, S. cerevisiae strains with the capability of utilizing both glucose and xylose in the presence of inhibitors such as furfural are very important in industrial ethanol production. Under the synergistic conditions of transaldolase (TAL) and alcohol dehydrogenase (ADH) overexpression, S. cerevisiae MT8-1X/TAL–ADH was able to produce 1.3-fold and 2.3-fold more ethanol in the presence of 70 mM furfural than a TAL-expressing strain and a control strain, respectively. We also tested the strains' ability by mimicking industrial ethanol production from hemicellulosic hydrolysate containing fermentation inhibitors, and ethanol production was further improved by 16% when using MT8-1X/TAL–ADH compared to the control strain. Transcript analysis further revealed that besides the pentose phosphate pathway genes TKL1 and TAL1, ADH7 was also upregulated in response to furfural stress, which resulted in higher ethanol production compared to the TAL-expressing strain. The improved capability of our modified strain was based on its capacity to more quickly reduce furfural in situ resulting in higher ethanol production. The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production. 2014-03-15T07:42:49Z 2014-03-15T07:42:49Z 2014-02 Article Journal of Bioscience and Bioengineering, vol.117 (2), 2014, pages 165–169 1389-1723 http://dspace.unimap.edu.my:80/dspace/handle/123456789/32778 http://www.sciencedirect.com/science/article/pii/S1389172313002697 http://dx.doi.org/10.1016/j.jbiosc.2013.07.007 en Elsevier |
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Furfural TAL1 ADH1 Overexpression Hemicellulosic hydrolysate Xylose Saccharomyces cerevisiae Bioethanol |
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Furfural TAL1 ADH1 Overexpression Hemicellulosic hydrolysate Xylose Saccharomyces cerevisiae Bioethanol Hasunuma, Tomohisa Ku Syahidah, Ku Ismail Nambu, Yumiko Kondo, Akihiko Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
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Link to publisher's homepage at http://www.elsevier.com |
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hasunuma@port.kobe-u.ac.jp |
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hasunuma@port.kobe-u.ac.jp Hasunuma, Tomohisa Ku Syahidah, Ku Ismail Nambu, Yumiko Kondo, Akihiko |
| format |
Article |
| author |
Hasunuma, Tomohisa Ku Syahidah, Ku Ismail Nambu, Yumiko Kondo, Akihiko |
| author_sort |
Hasunuma, Tomohisa |
| title |
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
| title_short |
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
| title_full |
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
| title_fullStr |
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
| title_full_unstemmed |
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
| title_sort |
co-expression of tal1 and adh1 in recombinant xylose-fermenting saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural |
| publisher |
Elsevier |
| publishDate |
2014 |
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http://dspace.unimap.edu.my:80/dspace/handle/123456789/32778 |
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