Optimization of process conditions for lipase production from rice bran by solid-state fermentation
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Universiti Malaysia Perlis (UniMAP)
2014
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| Online Access: | http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37597 |
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my.unimap-375972019-05-02T08:36:43Z Optimization of process conditions for lipase production from rice bran by solid-state fermentation Nur Adila, Ahmad Mohamad Fahrurrazi Tompang Rice bran lipase Lipases Rice bran Solid state fermentation (SSF) Access is limited to UniMAP community. Lipases have potential application in different field of natural science. this study describes the production of lipases by solid – state fermentation by utilizing rice bran. Rice bran was used as the substrate for screening two funguses that is Aspergillus niger and Rhizopous oligosporus and also defined suitable incubation time for lipase production by solid- state fermentation. The best producer, Rhizopous oligosporous, was used for optimizing the process parameters for enzyme production for 6 days. The effects of the experimental variables on lipase activity had been optimized by using central composite design (CCD). Interactions were studied for three different conditions that is initial pH, temperature and moisture content for fermentation. Fermentation was then carried out under optimized conditions to obtain the lipase activity using lipase assay. Fermentation with 65 % initial moisture content and pH 6.5 of mineral media at temperature 35 oc for 6 days was found to be optimum for enzymes secretion by the fermenting organism that is 0.1753 U/mL. Biomass estimation also determined after fermentation to show growth profile for Rhizopous oligosporus. the growth profile showed that day six is the optimum growth for Rhizopous oligosporus where after day six the growth is slowly decreased. For validation of experiment, the suggested value for initial pH is 6.48, temperature is 36.68 oc and moisture content is 64.9 %. the lipases activity for validation of experiment is 0.162676 U/mL. 2014-11-04T01:51:06Z 2014-11-04T01:51:06Z 2013-05 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37597 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering |
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Malaysia |
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Universiti Malaysia Perlis |
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| language |
English |
| topic |
Rice bran lipase Lipases Rice bran Solid state fermentation (SSF) |
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Rice bran lipase Lipases Rice bran Solid state fermentation (SSF) Nur Adila, Ahmad Optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| description |
Access is limited to UniMAP community. |
| author2 |
Mohamad Fahrurrazi Tompang |
| author_facet |
Mohamad Fahrurrazi Tompang Nur Adila, Ahmad |
| format |
Learning Object |
| author |
Nur Adila, Ahmad |
| author_sort |
Nur Adila, Ahmad |
| title |
Optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| title_short |
Optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| title_full |
Optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| title_fullStr |
Optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| title_full_unstemmed |
Optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| title_sort |
optimization of process conditions for lipase production from rice bran by solid-state fermentation |
| publisher |
Universiti Malaysia Perlis (UniMAP) |
| publishDate |
2014 |
| url |
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37597 |
| _version_ |
1643806166687416320 |