Optimization of process conditions of lipase production from coconut dregs by solid state fermentation

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Bibliographic Details
Main Author:	Nittiyah, Vasudevan
Other Authors:	Mohamad Fahrurrazi Tompang
Format:	Learning Object
Language:	English
Published:	Universiti Malaysia Perlis (UniMAP) 2014
Subjects:	Lipases Solid state fermentation (SSF) Coconut dregs Lipase production
Online Access:	http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37625
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Institution:	Universiti Malaysia Perlis
Language:	English

id	my.unimap-37625
record_format	dspace
spelling	my.unimap-376252014-11-05T02:12:00Z Optimization of process conditions of lipase production from coconut dregs by solid state fermentation Nittiyah, Vasudevan Mohamad Fahrurrazi Tompang Lipases Solid state fermentation (SSF) Coconut dregs Lipase production Access is limited to UniMAP community. Lipases comprise a group of hydrolytic enzymes which catalyze the hydrolysis and synthesis of triacylglycerides in the oil water interface. Screening of microorganism for extracellular lipase production from coconut dregs was conducted by using Aspergillus niger and Rhizopus oligosporus. The result shows that Rhizopus oligosporus able to produce higher lipase, 0.1527 U/mL compared to Aspergillus niger and thus it was chosen for further analysis. Process conditions such as pH, temperature and moisture content which influence the growth and production of lipase were studied. Central-Composite Design (CCD) of Response Surface approach was used to analyze the results and the optimum conditions for lipase production was determined. According to CCD, the optimum process conditions for Rhizopus oligosporus to produce lipase were as follow: 34.8 ˚C, pH 5.24 and 62 % of moisture content. Validation process was carried out for 10 days under these conditions and it gives the maximum lipase activity at day 6 (0.2281 U/mL) . The standard error between optimization and validation was 2.23 %. 2014-11-05T02:12:00Z 2014-11-05T02:12:00Z 2013-05 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37625 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
building	UniMAP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Perlis
content_source	UniMAP Library Digital Repository
url_provider	http://dspace.unimap.edu.my/
language	English
topic	Lipases Solid state fermentation (SSF) Coconut dregs Lipase production
spellingShingle	Lipases Solid state fermentation (SSF) Coconut dregs Lipase production Nittiyah, Vasudevan Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
description	Access is limited to UniMAP community.
author2	Mohamad Fahrurrazi Tompang
author_facet	Mohamad Fahrurrazi Tompang Nittiyah, Vasudevan
format	Learning Object
author	Nittiyah, Vasudevan
author_sort	Nittiyah, Vasudevan
title	Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_short	Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_full	Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_fullStr	Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_full_unstemmed	Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_sort	optimization of process conditions of lipase production from coconut dregs by solid state fermentation
publisher	Universiti Malaysia Perlis (UniMAP)
publishDate	2014
url	http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37625
_version_	1643798525360734208

Optimization of process conditions of lipase production from coconut dregs by solid state fermentation

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