Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain

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Main Author:	Nur Atiqah, Mad Arif
Other Authors:	Mohamad Fahrurrazi Tompang
Format:	Learning Object
Language:	English
Published:	Universiti Malaysia Perlis (UniMAP) 2014
Subjects:	Sugarcane molasses Polyhydroxyalkanoate (PHA) Ralstonia eutropha Fermentation
Online Access:	http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37626
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Institution:	Universiti Malaysia Perlis
Language:	English

id	my.unimap-37626
record_format	dspace
spelling	my.unimap-376262014-11-05T02:18:49Z Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain Nur Atiqah, Mad Arif Mohamad Fahrurrazi Tompang Sugarcane molasses Polyhydroxyalkanoate (PHA) Ralstonia eutropha Fermentation Access is limited to UniMAP community. A study of polyhydroxyalkanoate (PHA) production was conducted using pretreatment of sugarcane molasses as main substrate to obtain low-cost medium for fermentation process. Ralstonia eutropha strain was used to produce polyhydroxyalkanoate in the nutrient limitation conditions. The study involved determination of significance interaction between different parameter which is sugarcane molasses, ammonium sulfate and urea using Central Composite Design in order to optimize the PHA concentration. Two-stage fermentation techniques were used to obtained high yield of PHA fermentation. First stage fermentation involved bacteria growth phase in a nutrient rich medium and second stage fermentation involved culturing bacteria strain in the limited nutrient medium. The fermentation process was incubated at fixed set point values of agitation speed, pH, fermentation period and temperature. The highest PHA composition recorded was 72.03 % at 2 % sugarcane molasses, 0.21 % ammonium sulfate and 1 % urea concentrations. 2014-11-05T02:18:49Z 2014-11-05T02:18:49Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37626 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
building	UniMAP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Perlis
content_source	UniMAP Library Digital Repository
url_provider	http://dspace.unimap.edu.my/
language	English
topic	Sugarcane molasses Polyhydroxyalkanoate (PHA) Ralstonia eutropha Fermentation
spellingShingle	Sugarcane molasses Polyhydroxyalkanoate (PHA) Ralstonia eutropha Fermentation Nur Atiqah, Mad Arif Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
description	Access is limited to UniMAP community.
author2	Mohamad Fahrurrazi Tompang
author_facet	Mohamad Fahrurrazi Tompang Nur Atiqah, Mad Arif
format	Learning Object
author	Nur Atiqah, Mad Arif
author_sort	Nur Atiqah, Mad Arif
title	Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_short	Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_full	Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_fullStr	Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_full_unstemmed	Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_sort	optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
publisher	Universiti Malaysia Perlis (UniMAP)
publishDate	2014
url	http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37626
_version_	1643798525663772672

Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain

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