Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
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Universiti Malaysia Perlis (UniMAP)
2015
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| Online Access: | http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465 |
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my.unimap-384652015-01-11T08:30:20Z Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor Tan, Chee Kang Dr. A.K.M Shafiqul Islam Biosensor Glucose oxidase (GOX) enzyme Nanotubes Carbon paste electrode Access is limited to UniMAP community. A hybrid sol-gel carbon nanotubes composite has been developed and used for surface covalent bonding of enzyme for biosensing application. The biosensor was developed by covalent immobilization of glucose oxidase enzyme on sol gel modified electrode with ferrocene via carbodiimide reaction. The presence of amine groups provided by APTMOS in sol gel controlled the covalent bonding sites for enzyme. The experiment conditions such as amount of enzyme and pH in working buffer was studied by using Response Surface Methodology (RSM) through Central Composite Design (CCD) of Design Expert software. The biosensor showed maximum current response at 20 U/mL of immobilized glucose oxidase at the optimum pH value of working buffer of pH 7. The calibration curve of GOx biosensor was linear in the range of 0.1 mM to 3 mM of glucose concentration with a linear equation and correlation coefficient of 0.9873. The performance of GOx biosensor was evaluated based on selectivity toward the interferences and stability, the GOx biosensor exhibited high selectivity behavior and good stability. 2015-01-11T08:30:20Z 2015-01-11T08:30:20Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering |
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Biosensor Glucose oxidase (GOX) enzyme Nanotubes Carbon paste electrode |
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Biosensor Glucose oxidase (GOX) enzyme Nanotubes Carbon paste electrode Tan, Chee Kang Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
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Access is limited to UniMAP community. |
| author2 |
Dr. A.K.M Shafiqul Islam |
| author_facet |
Dr. A.K.M Shafiqul Islam Tan, Chee Kang |
| format |
Learning Object |
| author |
Tan, Chee Kang |
| author_sort |
Tan, Chee Kang |
| title |
Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
| title_short |
Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
| title_full |
Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
| title_fullStr |
Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
| title_full_unstemmed |
Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
| title_sort |
immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor |
| publisher |
Universiti Malaysia Perlis (UniMAP) |
| publishDate |
2015 |
| url |
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465 |
| _version_ |
1643798767814574080 |