The use of lates calcarifer as a biomarker for heavy metals detection
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2016
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my.unimap-411142016-03-09T04:03:42Z The use of lates calcarifer as a biomarker for heavy metals detection Nursabrina, Mohd Hayat Mohd Khalizan Halizan, Sabullah Ariff, Khalid Farrah Aini, Dahalan Siti Aqlima, Ahmad Mohd Yunus, Shukor Nor Aripin, Shamaan Mohd Arif, Syed farrahaini@unimap.edu.my Biomarker Biosensor Cholinesterase Heavy metals Link to publisher's homepage at http://link.springer.com Fish are ubiquitous organisms that have many features that designate their potential as a biomarker of heavy metals pollution. Thus, an investigation was done to detect the effect of heavy metals on cholinesterase (ChE) activity from Lates calcarifer organs which were gill and muscle. Ammonium sulphate precipitation was performed along with ion exchange chromatography to purify the enzyme. In the substrate specificity study, ChE from L. calcarifer gills was capable of breaking down acetylthiocholine iodide (ATC) at a faster rate compared to the other two synthetic substrates, which are butyrylthiocholine iodide (BTC) and propionylthiocholine iodide (PTC). In contrast, the muscle ChE has a higher affinity towards PTC. The maximum activity of ChE observed at the temperature ranging from 20 to 30 °C in Tris–HCl buffer pH 8. ChE from the two organs of L. calcarifer showed an inhibitive reaction towards heavy metals, but with different effects. ATC from gills showed 50 % inhibition by Cu, Hg and Pb, while PTC from muscle showed 50 % inhibition by Pb. The variation of inhibitory effect that was shown by ChE from L. calcarifer organs can be further studied in designing a biosensor kit that is sensitive towards heavy metal. 2016-03-09T04:01:39Z 2016-03-09T04:01:39Z 2016-01-21 Article Rendiconti Lincei, 2016, pages 1-10 1120-6349 1720-0776 (online) http://link.springer.com/article/10.1007/s12210-015-0501-7 http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41114 10.1007/s12210-015-0501-7 en Springer Milan |
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Universiti Malaysia Perlis |
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| language |
English |
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Biomarker Biosensor Cholinesterase Heavy metals |
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Biomarker Biosensor Cholinesterase Heavy metals Nursabrina, Mohd Hayat Mohd Khalizan Halizan, Sabullah Ariff, Khalid Farrah Aini, Dahalan Siti Aqlima, Ahmad Mohd Yunus, Shukor Nor Aripin, Shamaan Mohd Arif, Syed The use of lates calcarifer as a biomarker for heavy metals detection |
| description |
Link to publisher's homepage at http://link.springer.com |
| author2 |
farrahaini@unimap.edu.my |
| author_facet |
farrahaini@unimap.edu.my Nursabrina, Mohd Hayat Mohd Khalizan Halizan, Sabullah Ariff, Khalid Farrah Aini, Dahalan Siti Aqlima, Ahmad Mohd Yunus, Shukor Nor Aripin, Shamaan Mohd Arif, Syed |
| format |
Article |
| author |
Nursabrina, Mohd Hayat Mohd Khalizan Halizan, Sabullah Ariff, Khalid Farrah Aini, Dahalan Siti Aqlima, Ahmad Mohd Yunus, Shukor Nor Aripin, Shamaan Mohd Arif, Syed |
| author_sort |
Nursabrina, Mohd Hayat |
| title |
The use of lates calcarifer as a biomarker for heavy metals detection |
| title_short |
The use of lates calcarifer as a biomarker for heavy metals detection |
| title_full |
The use of lates calcarifer as a biomarker for heavy metals detection |
| title_fullStr |
The use of lates calcarifer as a biomarker for heavy metals detection |
| title_full_unstemmed |
The use of lates calcarifer as a biomarker for heavy metals detection |
| title_sort |
use of lates calcarifer as a biomarker for heavy metals detection |
| publisher |
Springer Milan |
| publishDate |
2016 |
| url |
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41114 |
| _version_ |
1643799577597313024 |