Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA

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Main Authors: Samla, G, Gan, K. B., Then, S. M.
Other Authors: sam2011sj@yahoo.com.
Format: Article
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
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Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/49814
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Institution: Universiti Malaysia Perlis
Language: English
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spelling my.unimap-498142017-11-21T01:25:46Z Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA Samla, G Gan, K. B. Then, S. M. sam2011sj@yahoo.com. DNA extraction SPME Whole blood Micro-device Link to publisher's homepage at http://ijneam.unimap.edu.my/ Deoxyribonucleic acid (DNA) extraction or sample preparation from biological sample (whole blood) for downstream process on microfluidic platform has been widely studied due to its crucial role in clinical diagnostic and genetic analysis. Sample preparation can be complicated since blood comprises complex matrices and repeated blood pricking from patients can be affected their health. Thus, introduction of solid-phase microextraction (SPME) method would be suitable to isolate, fractionate and concentrate the analyte from complex sample matrices and implemented on microfluidic device with small amount of sample. SPME is an effective sample preparation method by chemical lysis followed by purification mostly by using silica-based platform developed based on direct absorption into silica resins and desorption of analyte from silica resins. 2017-09-28T07:35:16Z 2017-09-28T07:35:16Z 2017 Article International Journal of Nanoelectronics and Materials, vol.10 (1), 2017, pages 75-92 1985-5761 (Printed) 1997-4434 (Online) http://dspace.unimap.edu.my:80/xmlui/handle/123456789/49814 en Universiti Malaysia Perlis (UniMAP)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic DNA extraction
SPME
Whole blood
Micro-device
spellingShingle DNA extraction
SPME
Whole blood
Micro-device
Samla, G
Gan, K. B.
Then, S. M.
Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA
description Link to publisher's homepage at http://ijneam.unimap.edu.my/
author2 sam2011sj@yahoo.com.
author_facet sam2011sj@yahoo.com.
Samla, G
Gan, K. B.
Then, S. M.
format Article
author Samla, G
Gan, K. B.
Then, S. M.
author_sort Samla, G
title Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA
title_short Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA
title_full Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA
title_fullStr Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA
title_full_unstemmed Solid phase microextraction based micro-device for extraction of PCR amplifiable DNA
title_sort solid phase microextraction based micro-device for extraction of pcr amplifiable dna
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/49814
_version_ 1643802825687302144