Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface
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Universiti Malaysia Perlis (UniMAP)
2020
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my.unimap-649242020-06-15T01:35:24Z Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface Nurul Atiqah, Ahmad Ruslinda, A. Rahim Bohuslav, Rezek Alexander, Kromka Nur Syakimah, Ismail Subash Chandra Bose Gopinath Tibor, Izak Vaclav, Prochazka Fatin Nabilah, Mohd Faudzi Azrul Syafiq, Zainol Abidin Nur Nasyifa, Mohd Maidizn ruslinda@unimap.edu.my Aptamer Electrolyte-gated Field Effect Transistor HIV-1 Tat Nanocrystalline Diamonds Link to publisher's homepage at http://ijneam.unimap.edu.my Nanocrystalline diamonds have recently gained great attention to circumvent the current hurdles, with their appealing properties such as high-surface-area to volume ratio, low-background current, wide potential window, biocompatibility, and chemical stability. The nanocrystalline diamonds electrolyte-gated field-effect transistor (NCD-EGFET) can operate directly in solution without involving gate oxides in bringing the hydrogen-tethered moieties and facilitates the p-type surface conductivity. This research investigated on Trans-activator of transcription (Tat) protein; a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis. 2020-06-15T01:35:24Z 2020-06-15T01:35:24Z 2020-04 Article International Journal of Nanoelectronics and Materials, vol.13(2), 2020, pages 295-306 1985-5761 (Printed) 1997-4434 (Online) http://dspace.unimap.edu.my:80/xmlui/handle/123456789/64924 http://ijneam.unimap.edu.my en Universiti Malaysia Perlis (UniMAP) |
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Aptamer Electrolyte-gated Field Effect Transistor HIV-1 Tat Nanocrystalline Diamonds |
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Aptamer Electrolyte-gated Field Effect Transistor HIV-1 Tat Nanocrystalline Diamonds Nurul Atiqah, Ahmad Ruslinda, A. Rahim Bohuslav, Rezek Alexander, Kromka Nur Syakimah, Ismail Subash Chandra Bose Gopinath Tibor, Izak Vaclav, Prochazka Fatin Nabilah, Mohd Faudzi Azrul Syafiq, Zainol Abidin Nur Nasyifa, Mohd Maidizn Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface |
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Link to publisher's homepage at http://ijneam.unimap.edu.my |
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ruslinda@unimap.edu.my |
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ruslinda@unimap.edu.my Nurul Atiqah, Ahmad Ruslinda, A. Rahim Bohuslav, Rezek Alexander, Kromka Nur Syakimah, Ismail Subash Chandra Bose Gopinath Tibor, Izak Vaclav, Prochazka Fatin Nabilah, Mohd Faudzi Azrul Syafiq, Zainol Abidin Nur Nasyifa, Mohd Maidizn |
| format |
Article |
| author |
Nurul Atiqah, Ahmad Ruslinda, A. Rahim Bohuslav, Rezek Alexander, Kromka Nur Syakimah, Ismail Subash Chandra Bose Gopinath Tibor, Izak Vaclav, Prochazka Fatin Nabilah, Mohd Faudzi Azrul Syafiq, Zainol Abidin Nur Nasyifa, Mohd Maidizn |
| author_sort |
Nurul Atiqah, Ahmad |
| title |
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface |
| title_short |
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface |
| title_full |
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface |
| title_fullStr |
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface |
| title_full_unstemmed |
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface |
| title_sort |
nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing hiv-1 tat on hydrogen-terminated surface |
| publisher |
Universiti Malaysia Perlis (UniMAP) |
| publishDate |
2020 |
| url |
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/64924 |
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1670805991505002496 |