Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis

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Main Author:	Ong, Yee Fen
Other Authors:	School of Bioprocess Engineering
Format:	Other
Language:	English
Published:	Universiti Malaysia Perlis (UniMAP) 2022
Subjects:	Antimicrobial Anthracnose Disease Bacillus Subtilis Aloe vera Colletotrichum Capsici Antifungal
Online Access:	http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73291
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Institution:	Universiti Malaysia Perlis
Language:	English

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spelling	my.unimap-732912022-01-11T07:26:16Z Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis Ong, Yee Fen School of Bioprocess Engineering Antimicrobial Anthracnose Disease Bacillus Subtilis Aloe vera Colletotrichum Capsici Antifungal Access is limited to UniMAP community. Nowadays, overuse of chemical fungicide has side effect which is harmful to human consumption, causes environmental problem, and destroy the antagonist microorganisms. Overuse of antibiotic also resulted to multi drug-resistant (MDR), where antibiotics become ineffective against the bacterial strains. Therefore, it is important to develop natural fungicide and bactericide from plant extract. For the current study, chilli anthracnose fungus, Colletotrichum capsici and bacteria Bacillus subtilis were used for antifungal and antibacterial activity. C. capcisi was isolated from chilli and its morphology was identified by its greyish-white colony colour, fluffy cottony look on PDA, rings of acervuli and curved conidia. Antifungal activity was demonstrated by using different concentration Aloe vera extract (1000 ppm to 5000 ppm). Aloe vera has low inhibition against C. capsici at every concentration which were 1.17 % at 1000 ppm and 7.20 % at 5000ppm. As for the antibacterial activity, different concentrations of Aloe vera extracts (1.25 mg/ml until 200 mg/ml) were tested on B. subtilis by using disc diffusion method. Aloe vera has high inhibition against B. subtilis at 200 mg/ml which was 21.33 mm while there is no inhibition zone at 1.25 mg/ml. B. subtilis proved that MIC value is 2.5 mg/ml. The phytochemical test confirmed the presence of terpenoids, flavonoids, tannins and steroids. In conclusion, Aloe vera can be biological fungicide to replace chemical fungicide against C. capsici but research still need to carry out to enhance the efficiency towards C.capsici. On the other hand, Aloe vera is suitable to be commercialized as natural bactericide. 2022-01-11T07:26:16Z 2022-01-11T07:26:16Z 2017-06 Other http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73291 en Universiti Malaysia Perlis (UniMAP)
institution	Universiti Malaysia Perlis
building	UniMAP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Perlis
content_source	UniMAP Library Digital Repository
url_provider	http://dspace.unimap.edu.my/
language	English
topic	Antimicrobial Anthracnose Disease Bacillus Subtilis Aloe vera Colletotrichum Capsici Antifungal
spellingShingle	Antimicrobial Anthracnose Disease Bacillus Subtilis Aloe vera Colletotrichum Capsici Antifungal Ong, Yee Fen Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis
description	Access is limited to UniMAP community.
author2	School of Bioprocess Engineering
author_facet	School of Bioprocess Engineering Ong, Yee Fen
format	Other
author	Ong, Yee Fen
author_sort	Ong, Yee Fen
title	Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis
title_short	Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis
title_full	Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis
title_fullStr	Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis
title_full_unstemmed	Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis
title_sort	assessing of aloe vera for antimicrobial against infected capsicum annuum by colletotrichum capsici for anthracnose disease and bacillus subtilis
publisher	Universiti Malaysia Perlis (UniMAP)
publishDate	2022
url	http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73291
_version_	1724610027368480768

Assessing of Aloe Vera for antimicrobial against infected Capsicum Annuum by Colletotrichum Capsici for Anthracnose disease and Bacillus Subtilis

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