Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia
A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to an...
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my.unimas.ir.101052021-07-14T17:13:59Z http://ir.unimas.my/id/eprint/10105/ Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia Son, Radu Y.K, Ho Samuel, Lihan Yuherman, - G., Rusul R. M., Yasin J., Khair N., Elhadi QR Microbiology A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antibiotics, plasmid pro®les and random ampli®ed polymorphic DNA (RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical antibiotic resistance patterns of the isolates may indicate that they share a common mode of developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing showed that all strains tested originated from high risk contamination. Plasmid pro®le analysis by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5 O1 serotypes) with sizes ranging 1±3±4±6 MDa. The CT gene was detected in all clinical isolates but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non- O1 strains was investigated by RAPD ®ngerprinting with four primers. The four primers generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging from !250 to 4500 bp. The RAPD pro®les revealed a wide variability and no correlation with the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have signi®cant public health implications. Cambridge University Press 1999 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10105/1/Lihan.pdf Son, Radu and Y.K, Ho and Samuel, Lihan and Yuherman, - and G., Rusul and R. M., Yasin and J., Khair and N., Elhadi (1999) Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia. Epidemiology and Infection, 123 (2). pp. 225-232. https://www.researchgate.net/publication/231998329_Molecular_characterization_of_Vibrio_cholerae_O1_and_non-O1_from_human_and_environmental_sources_in_Malaysia |
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QR Microbiology Son, Radu Y.K, Ho Samuel, Lihan Yuherman, - G., Rusul R. M., Yasin J., Khair N., Elhadi Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
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A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water)
and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and
1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based
assays, resistance to antibiotics, plasmid pro®les and random ampli®ed polymorphic DNA
(RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical
antibiotic resistance patterns of the isolates may indicate that they share a common mode of
developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing
showed that all strains tested originated from high risk contamination. Plasmid pro®le analysis
by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5
O1 serotypes) with sizes ranging 1±3±4±6 MDa. The CT gene was detected in all clinical isolates
but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental
waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non-
O1 strains was investigated by RAPD ®ngerprinting with four primers. The four primers
generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging
from !250 to 4500 bp. The RAPD pro®les revealed a wide variability and no correlation with
the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have
signi®cant public health implications. |
format |
Article |
author |
Son, Radu Y.K, Ho Samuel, Lihan Yuherman, - G., Rusul R. M., Yasin J., Khair N., Elhadi |
author_facet |
Son, Radu Y.K, Ho Samuel, Lihan Yuherman, - G., Rusul R. M., Yasin J., Khair N., Elhadi |
author_sort |
Son, Radu |
title |
Molecular characterization of Vibrio cholerae O1 and
non-O1 from human and environmental sources in Malaysia |
title_short |
Molecular characterization of Vibrio cholerae O1 and
non-O1 from human and environmental sources in Malaysia |
title_full |
Molecular characterization of Vibrio cholerae O1 and
non-O1 from human and environmental sources in Malaysia |
title_fullStr |
Molecular characterization of Vibrio cholerae O1 and
non-O1 from human and environmental sources in Malaysia |
title_full_unstemmed |
Molecular characterization of Vibrio cholerae O1 and
non-O1 from human and environmental sources in Malaysia |
title_sort |
molecular characterization of vibrio cholerae o1 and
non-o1 from human and environmental sources in malaysia |
publisher |
Cambridge University Press |
publishDate |
1999 |
url |
http://ir.unimas.my/id/eprint/10105/1/Lihan.pdf http://ir.unimas.my/id/eprint/10105/ https://www.researchgate.net/publication/231998329_Molecular_characterization_of_Vibrio_cholerae_O1_and_non-O1_from_human_and_environmental_sources_in_Malaysia |
_version_ |
1706961315507994624 |