Campylobacter jejuni detection in proboscis monkey (Nasalis larvatus)

• Main Author: Julian, Bibi Jacquelyn
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/10505/8/Campylobacter%20jejuni%20Detection%20In%20Proboscis%20Monkey%20%28Nasalis%20larvatus%29%20%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/10505/
• Institution: Universiti Malaysia Sarawak

This study was done to detect the prevalence of Campylobacter jejuni in proboscis monkey’s fecal samples collected from Bako National Park (n = 5) and Samunsam Wildlife Sanctuary, Sarawak (n = 37). C. jejuni is commonly found in wildlife and domestic animals. The stool samples were enriched with Bolton broth (ISO 10272-1: 2006), followed by cultured on selective agar, modified charcoal cefoperazone deoxycholate agar (mCCDA) and were then sub-cultured on Bolton broth. Pure cultures of the isolated stool samples were tested with Gram staining for morphological identification. In this study, Boiling method protocol was used for purification of total DNA from the established pure cultures of the stool samples. All 42 established pure cultures of the stool samples gave no reaction with the PCR assay targeting hippuricase gene for species level detection of C. jejuni. However, high numbers of positive cultures were observed on the selective agar, mCCDA, which indicating C. jejuni positive colonies. Furthermore, morphological test on the cultures observed S-shaped rod characteristic. The prevalence of Campylobacter spp. detected in fecal samples collected from Samunsam Wildlife Sanctuary (86.486%) was higher than of Bako National Park (60%). Although the specific species of Campylobacter cannot be identified via PCR approach, the presence of colonies on mCCDA with high prevalence indicates that proboscis monkey may be considered as a potential reservoir of Campylobacter spp.