Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus

Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of...

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Main Authors:	Henry Sum, Magdline Sia, Andrew, Anna
Format:	Article
Language:	English
Published:	Trade Science Inc. 2015
Subjects:	Q Science (General) QR355 Virology R Medicine (General)
Online Access:	http://ir.unimas.my/id/eprint/10669/1/Expression.pdf http://ir.unimas.my/id/eprint/10669/
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Institution:	Universiti Malaysia Sarawak
Language:	English

id	my.unimas.ir.10669
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spelling	my.unimas.ir.106692022-01-26T08:15:40Z http://ir.unimas.my/id/eprint/10669/ Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus Henry Sum, Magdline Sia Andrew, Anna Q Science (General) QR355 Virology R Medicine (General) Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum Trade Science Inc. 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10669/1/Expression.pdf Henry Sum, Magdline Sia and Andrew, Anna (2015) Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus. Bio Technology - An Indian Journal, 11 (3). pp. 109-114. ISSN 0974-7435
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) QR355 Virology R Medicine (General)
spellingShingle	Q Science (General) QR355 Virology R Medicine (General) Henry Sum, Magdline Sia Andrew, Anna Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
description	Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum
format	Article
author	Henry Sum, Magdline Sia Andrew, Anna
author_facet	Henry Sum, Magdline Sia Andrew, Anna
author_sort	Henry Sum, Magdline Sia
title	Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
title_short	Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
title_full	Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
title_fullStr	Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
title_full_unstemmed	Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
title_sort	expression and evaluation of a 24-kda recombinant protein of the n-terminal e2 glycoprotein of chikungunya virus
publisher	Trade Science Inc.
publishDate	2015
url	http://ir.unimas.my/id/eprint/10669/1/Expression.pdf http://ir.unimas.my/id/eprint/10669/
_version_	1724078477149208576

Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus

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