Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA

An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed...

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Bibliographic Details
Main Author: Sing, Tung Teng
Format: E-Article
Language:English
Published: Springer Japan 2015
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Online Access:http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/10719/
http://link.springer.com/article/10.1007%2Fs12562-015-0902-7
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Institution: Universiti Malaysia Sarawak
Language: English
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Summary:An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1– 8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.