A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
Eusideroxylon zwageri is a tree of tropical rainforest zone and belongs to a Lauraceae family. It is one of the hardest timber species in Southeast Asia. The objective of this study was to determine the optimal culture medium for the induction of somatic embryogenesis f explants of E. zwageri....
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Main Authors: | , |
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Format: | E-Article |
Language: | English |
Published: |
IJAIR
2016
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/12877/1/A%20Preliminary%20Study%20on%20the%20Induction%20of%20Somatic%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/12877/ http://ijair.org/index.php/issues?view=publication&task=show&id=756 |
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Institution: | Universiti Malaysia Sarawak |
Language: | English |
Summary: | Eusideroxylon zwageri is a tree of tropical
rainforest zone and belongs to a Lauraceae family. It is one
of the hardest timber species in Southeast Asia. The objective
of this study was to determine the optimal culture medium
for the induction of somatic embryogenesis f
explants of E. zwageri. It was found that the globular somatic
embryos were induced in half strength of MS medium with
1.0, 1.5, 2.0 mg/L of BAP in combination with either 0.5 mg/L
of 2,4-D or NAA. The maturation of somatic embryos
obtained in half strength of MS medium with BAP, NAA and
GA3. The highest mean number of the induction of somatic
embryos up to the cotyledonary phase was observed in
culture medium containing 1.0 mg/L of BAP, 0.5 mg/L of
NAA in combination with 1.0 mg/L of GA
reported preliminary study was useful for further plantlet
regeneration of this species through induction of somatic
embryogenesis. |
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