Escherichia coli bactofection using Lipofectamine

• Main Authors: Kumaran, Narayanan, Choon, Weng Lee, Aurelian, Radu, Edmund, Ui Hang Sim
• Format: E-Article
• Language: English
• Published: Academic Press Inc. 2013
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/16310/1/Escherichia%20coli%20bactofection%20using%20Lipofectamine%20%28abstrak%29.pdf; http://ir.unimas.my/id/eprint/16310/; http://www.sciencedirect.com/science/journal/00032697
• Institution: Universiti Malaysia Sarawak

Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine impro...