Purification of an alpha amylase from Aspergillus flavus NSH9 and molecular characterization of its nucleotide gene sequence

• Main Authors: Karim, Kazi Muhammad Rezaul, Husaini, Ahmad, Sing, Ngieng Ngui, Sinang, Fazia Mohd, Roslan, Hairul Azman, Hussain, Hasnain
• Format: E-Article
• Language: English
• Published: Springer Verlag 2018
• Subjects: GE Environmental Sciences
• Online Access: http://ir.unimas.my/id/eprint/20197/1/Purification-of-an-alpha-amylase-from-Aspergillus-flavus-NSH9-and-molecular-characterization-of-its-nucleotide-gene-sequence_2018_3-Biotech%20-%20%28abstrak%29.pdf; http://ir.unimas.my/id/eprint/20197/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044659977&doi=10.1007%2fs13205-018-1225-z&partnerID=40&md5=2903f64fd085b4d479c098d1c29f9dfb
• Institution: Universiti Malaysia Sarawak

In this study, an alpha-amylase enzyme from a locally isolated Aspergillus flavus NSH9 was purified and characterized. The extracellular α-amylase was purified by ammonium sulfate precipitation and anion-exchange chromatography at a final yield of 2.55-fold and recovery of 11.73%. The molecular mass...