Sequencing and Characterization of Complete Mitogenome DNA for Rasbora spp. with Phylogenetic Consideration

• Main Author: Cynthia Keranti, Kamar
• Format: Thesis
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2019
• Subjects: Q Science (General); QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/26222/1/Cynthia%20Keranti%20Anak%20Kamar%20ft.pdf; http://ir.unimas.my/id/eprint/26222/
• Institution: Universiti Malaysia Sarawak

Rasbora are freshwater fishes that belongs to Cyprinidae family. Rasbora species share similar morphology and are closely related which makes them difficult to be differentiated between species. In this study, the morphometry analysis and mitochondrial DNA were used to distinguish the species and able to resolve the phylogenetic relationship between these species. A total of 58 rasbora specimens from four species (Rasbora sumatrana (20), Rasbora hobelmani (11), Rasbora tornieri (20), and Rasbora borapetensis (7)) were individually identified using morphometric analysis. The mitochondrial DNA were amplified by using the long polymerase chain reaction (PCR). Four sets of primer were designed for partial fragment of mitochondrial genome. The newly paired primer were made from these 4 sets of primer for complete fragments of mitochondrial genome. The complete mitochondrial DNA of 4 Rasbora species consists of 22 tRNA genes, 13 protein coding genes, and 2 rRNA genes with sequence full length of 16,539 bp (Rasbora sumatrana), 16,541 bp (Rasbora hobelmani), 16,573 bp (Rasbora tornieri), and 16,520 bp (Rasbora borapetensis) respectively. The phylogenetic position of these 4 Rasbora species was evaluated by using concatenated 13 protein coding genes of complete mitochondrial sequence. The morphometry and mitochondrial DNA study has successfully correlated these 4 Rasbora species by using discriminant function analysis and sequencing respectively. In conclusions, the molecular work produce more reliable results than morphometric study in identifying the species as the resolution of the species phylogeny can be observed.