Characterization and expression in Pichia pastoris of a raw starch degrading glucoamylase (GA2) derived from Aspergillus flavus NSH9

• Main Authors: Kazi Muhammad, Rezaul Karim, Ahmad, Husaini, Ngieng, Ngui Sing, Tasmia, Tanim, Fazia, Mohd Sinang, Hasnain, Hussain, Md Anowar, Hossain, Hairul Azman, Roslan
• Format: Article
• Published: Elsevier Inc. 2019
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/27450/; https://www.sciencedirect.com/science/article/pii/S1046592819300440?via%3Dihub; https://doi.org/10.1016/j.pep.2019.105462
• Institution: Universiti Malaysia Sarawak

The Aspergillus flavus NSH9 gene, encoding a pH and thermostable glucoamylase with a starch binding domain (SBD), was expressed in Pichia pastoris to produce recombinant glucoamylase (rGA2). The full-length glucoamylase gene (2039 bp), and cDNA (1839 bp) encode a 612 amino acid protein most similar...