In Vitro Mutagenesis of Neolamarckia macropylla using Ethyl Methanesulphonate (EMS)

• Main Author: Nurhidayu, Binti Mohd Sobri
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2017
• Subjects: Q Science (General); QH301 Biology
• Online Access: http://ir.unimas.my/id/eprint/27787/2/Nurhidayu.pdf; http://ir.unimas.my/id/eprint/27787/
• Institution: Universiti Malaysia Sarawak

This study was conducted to determine the optimum concentration and duration of ethyl methanesulphonate (EMS) treatment on callus morphogenesis and growth ofNeolamarckia macrophylla. The nodal-derived callus were soaked with various concentration of EMS, double distilled water as control treatment, 0.1, 0.3 and 0.6% (v/v) for 15,30 and 45 min. Then, the callus was rinsed 3 times with sterile double distilled water and Gamborg B5 liquid media and was transferred to propagation medium for 20 days and regeneration medium for next 20 days. This experiment was carried out with completely randomized design with two factors EMS concentration and soaking duration consist of3 replicates and each replicate comprise 3 nodal-derived callus. Result showed that survivability of callus percentage was decreased with the increase of EMS doses. Analysis variance was conducted, there was a significant effect of both EMS concentration and soaking duration on callus survivability. Post hoc test using Duncan's Multiple Range test stated that 0.1 % EMS was significant different compared to 0.3% and 0.6% while for soaking duration 15 minutes treatment was significantly different compared to 30 and 45 minutes treatment. Among the callus of N. macrophylla higher survival rate was observed at 0.1 % for 15,30 and 45 min. However, the maximum root length was observe from callus subjected to 0.6% for 30 min treatment. In addition, the treated callus with 0.1 % for 30 min gave the biggest bulblet size. Potential mutant roots with irregular development of roots, bulge-like structure roots and larger diameter of primary roots were observed. Confirmation of potential mutant roots is required for future studies up to molecular level.