Inflammation and Vascular Calcification Causing Effects of Oxidized HDL are Attenuated by Adiponectin in Human Vascular Smooth Muscle Cells
The role of oxidized high- density lipoprotein (oxHDL) and the protective effects of adiponectin in terms of vascular calcification is not well-established. This study was conducted to investigate the effects of oxHDL with regard to inflammation and vascular calcification and to determine the prote...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
2019
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/28297/1/Gabriele%20Ruth.pdf http://ir.unimas.my/id/eprint/28297/ http://ijmcmed.org/article-1-1059-en.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | The role of oxidized high- density lipoprotein (oxHDL) and the protective effects of adiponectin in terms of
vascular calcification is not well-established. This study was conducted to investigate the effects of oxHDL with regard to inflammation and vascular calcification and to determine the protective role of adiponectin in
attenuating the detrimental effects of oxHDL. Cell viability, mineralization, and calcification assays were
conducted to optimize the concentration of oxHDL. Then, human vascular smooth muscle cells (HAoVSMCs)
were incubated with β-glycerophosphate, HDL, oxHDL, adiponectin, or the combination of oxHDL with
adiponectin for 24 h. Protein expression of IL-6, TNF-α, osterix, RUNX2, ALP, type 1 collagen, osteopontin,
osteocalcin, WNT-5a, NF-ĸβ(p65), cAMP and STAT-3 were measured by ELISA kits. OxHDL induced vascular
calcification by promoting the formation of mineralization nodules and calcium deposits in HAoVSMCs. This was accompanied by an increased secretion of IL-6, osterix, WNT-5a and NF-ĸβ (p65). Interestingly, these
detrimental effects of oxHDL were suppressed by adiponectin. Besides, incubation of adiponectin alone on
HAoVSMCs showed a reduction of inflammatory cytokines, osteoblastic markers (RUNX2, osterix and osteopontin), WNT-5a and NF-ĸβ (p65). This study exhibits the ability of oxHDL in inducing inflammation and vascular calcification and these detrimental effects of oxHDL can be attenuated by adiponectin. |
|---|