Molecular phylogenetic of two Bornean Microchiroptera genera Hipposideros and Myotis and phylogeography of Hipposideros cervinus in Sabah and Sarawak

• Main Author: Paul, Imelda Vivian
• Format: Thesis
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2007
• Subjects: QL Zoology
• Online Access: http://ir.unimas.my/id/eprint/28814/1/Imelda%20Vivian%20Paul%20ft.pdf; http://ir.unimas.my/id/eprint/28814/
• Institution: Universiti Malaysia Sarawak

Phylogenetic relationships on Microchiroptera have previously been constructed based upon morphological and behavioral data but to date no one has investigated those relationships using molecular characters. This study reports the results of molecular analysis using sequences from two mitochondrial genes (12S rRNA and 16S rRNA) on two genera Hipposideros and Myotis of the Bornean Microchiroptera and also the phylogeography of H cervinus species in Sabah and Sarawak. Phylogenetic analysis of the genus Hipposideros was mostly concordant with those of Payne et a1 (1985) and Corbet and Hill (1992) especially regarding the sister relationships between H bicolor and Hater and between H larvatus and H diadema. The ML tree obtained also gave supports for the seven taxonomic species groups of Hipposideros proposed by Hill (1963). Unfortunately, phylogenetic analysis of the genus Myotis could not be fully resolved especially the interspecies relationship within this genus. There was also no support for the subdivision of genus Myotis into two subgenus; Selysius and Leuconoe. The only strong support was the close relationship between M horsfieldii and M hasseltii. This present study also utilised PCR-RFLP approach in exammmg the phylogenetic relationships among the species in the genus Hipposlderos. Although, there are some disagreements in the phylogenetic tree obtained if compared to sequence analysis, especially regarding the sister relationship but this method had successfully discovered two enzymes HindIII and KpnII as being species specific for H dyacorum and Hater.