Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae)
Thirteen isolates of Prorocentrum species were established from the coral reefs of Perhentian Islands Marine Park, Malaysia and underwent morphological observations and molecular characterization. Six species were found: P. caipirignum, P. concavum, P. cf. emarginatum, P. lima, P. mexicanum and a ne...
Saved in:
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier B.V.
2019
|
Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/29836/1/Taxonomy%20and%20toxicity.pdf http://ir.unimas.my/id/eprint/29836/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062861422&doi=10.1016%2fj.hal.2019.01.007&partnerID=40&md5=4c699f36a40c743c423acd36e6d25147 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Malaysia Sarawak |
Language: | English |
id |
my.unimas.ir.29836 |
---|---|
record_format |
eprints |
spelling |
my.unimas.ir.298362022-09-29T02:25:50Z http://ir.unimas.my/id/eprint/29836/ Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) Lim, Z.F., Luo, Z., Lee, L.K., Hii, K.S., Teng, Sing Tung Chan, L.L., Chomérat, N., Krock, B., Gu, H., Lim, P.T., Leaw, C.P. Q Science (General) S Agriculture (General) Thirteen isolates of Prorocentrum species were established from the coral reefs of Perhentian Islands Marine Park, Malaysia and underwent morphological observations and molecular characterization. Six species were found: P. caipirignum, P. concavum, P. cf. emarginatum, P. lima, P. mexicanum and a new morphotype, herein designated as P. malayense sp. nov. Prorocentrum malayense, a species closely related to P. leve, P. cf. foraminosum, P. sp. aff. foraminossum, and P. concavum (Clade A sensu Chomérat et al. 2018), is distinguished from its congeners as having larger thecal pore size and a more deeply excavated V-shaped periflagellar area. Platelet arrangement in the periflagellar area of P. malayense is unique, with the presence of platelet 1a and 1b, platelet 2 being the most anterior platelet, and a broad calabash-shaped platelet 3. The species exhibits consistent genetic sequence divergences for the nuclear-encoded large subunit ribosomal RNA gene (LSU rDNA) and the second internal transcribed spacer (ITS2). The phylogenetic inferences further confirmed that it represents an independent lineage, closely related to species in Clade A sensu Chomérat et al. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of compensatory base changes (CBCs). Toxicity analysis showed detectable levels of okadaic acid in P. lima (1.0–1.6 pg cell ˗1 ) and P. caipirignum (3.1 pg cell ˗1 ); this is the first report of toxigenic P. caipirignum in the Southeast Asian region. Other Prorocentrum species tested, including the new species, however, were below the detection limit. © 2019 Elsevier B.V. 2019-03 Article PeerReviewed text en http://ir.unimas.my/id/eprint/29836/1/Taxonomy%20and%20toxicity.pdf Lim, Z.F., and Luo, Z., and Lee, L.K., and Hii, K.S., and Teng, Sing Tung and Chan, L.L., and Chomérat, N., and Krock, B., and Gu, H., and Lim, P.T., and Leaw, C.P. (2019) Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae). Harmful Algae, 83. pp. 95-108. ISSN 1568-9883 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062861422&doi=10.1016%2fj.hal.2019.01.007&partnerID=40&md5=4c699f36a40c743c423acd36e6d25147 10.1016/j.hal.2019.01.007 |
institution |
Universiti Malaysia Sarawak |
building |
Centre for Academic Information Services (CAIS) |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sarawak |
content_source |
UNIMAS Institutional Repository |
url_provider |
http://ir.unimas.my/ |
language |
English |
topic |
Q Science (General) S Agriculture (General) |
spellingShingle |
Q Science (General) S Agriculture (General) Lim, Z.F., Luo, Z., Lee, L.K., Hii, K.S., Teng, Sing Tung Chan, L.L., Chomérat, N., Krock, B., Gu, H., Lim, P.T., Leaw, C.P. Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) |
description |
Thirteen isolates of Prorocentrum species were established from the coral reefs of Perhentian Islands Marine Park, Malaysia and underwent morphological observations and molecular characterization. Six species were found: P. caipirignum, P. concavum, P. cf. emarginatum, P. lima, P. mexicanum and a new morphotype, herein designated as P. malayense sp. nov. Prorocentrum malayense, a species closely related to P. leve, P. cf. foraminosum, P. sp. aff. foraminossum, and P. concavum (Clade A sensu Chomérat et al. 2018), is distinguished from its congeners as having larger thecal pore size and a more deeply excavated V-shaped periflagellar area. Platelet arrangement in the periflagellar area of P. malayense is unique, with the presence of platelet 1a and 1b, platelet 2 being the most anterior platelet, and a broad calabash-shaped platelet 3. The species exhibits consistent genetic sequence divergences for the nuclear-encoded large subunit ribosomal RNA gene (LSU rDNA) and the second internal transcribed spacer (ITS2). The phylogenetic inferences further confirmed that it represents an independent lineage, closely related to species in Clade A sensu Chomérat et al. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of compensatory base changes (CBCs). Toxicity analysis showed detectable levels of okadaic acid in P. lima (1.0–1.6 pg cell ˗1 ) and P. caipirignum (3.1 pg cell ˗1 ); this is the first report of toxigenic P. caipirignum in the Southeast Asian region. Other Prorocentrum species tested, including the new species, however, were below the detection limit. © 2019 |
format |
Article |
author |
Lim, Z.F., Luo, Z., Lee, L.K., Hii, K.S., Teng, Sing Tung Chan, L.L., Chomérat, N., Krock, B., Gu, H., Lim, P.T., Leaw, C.P. |
author_facet |
Lim, Z.F., Luo, Z., Lee, L.K., Hii, K.S., Teng, Sing Tung Chan, L.L., Chomérat, N., Krock, B., Gu, H., Lim, P.T., Leaw, C.P. |
author_sort |
Lim, Z.F., |
title |
Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) |
title_short |
Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) |
title_full |
Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) |
title_fullStr |
Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) |
title_full_unstemmed |
Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae) |
title_sort |
taxonomy and toxicity of prorocentrum from perhentian islands (malaysia), with a description of a non-toxigenic species prorocentrum malayense sp. nov. (dinophyceae) |
publisher |
Elsevier B.V. |
publishDate |
2019 |
url |
http://ir.unimas.my/id/eprint/29836/1/Taxonomy%20and%20toxicity.pdf http://ir.unimas.my/id/eprint/29836/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062861422&doi=10.1016%2fj.hal.2019.01.007&partnerID=40&md5=4c699f36a40c743c423acd36e6d25147 |
_version_ |
1745566052506402816 |