Molecular typing of Bacillus cereus isolated from sago processing mills in Sarawak.

• Main Author: Lesley Maurice, Bilung
• Format: Proceeding
• Language: English
• Published: 2017
• Subjects: S Agriculture (General)
• Online Access: http://ir.unimas.my/id/eprint/31063/1/Molecular%20Typing.pdf; http://ir.unimas.my/id/eprint/31063/
• Institution: Universiti Malaysia Sarawak

Bacillus cereus is an ubiquitous bacteria which is commonly found in soil and plant-based food. In this study, thirty-nine Bacillus cereus isolates were detected with the presence of hly gene by using specific PCR. These isolates were further characterized and differentiated by using the Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) to determine their genomic fingerprints. The ERIC-PCR generated several genetic profiles consisting of 1 to 7 bands with sizes in the range of 120 bp to 990 bp. Based on the dendrogram generated from the DNA fingerprinting profiles (ERIC-PCR), all of the isolates can be divided into 2 main clusters that is further divided into few sub-clusters. The heterogeneity of the isolates indicated the crosscontamination of Bacillus cereus occurred in sago processing mills in Sarawak.