Hot phenol extraction of total RNA from Thermoascus aurantiacus and the expression of a thermostable xylanase gene
Total RNA was successfully isolated using hot phenol extraction method. Three bands representing the 18S, 5.8S and 28S rRNA was observed. No heavy smearing was observed in the RNA band patterns, indicating low levels of polysaccharide contamination, when subjected to 1% agarose gel electrophoresis....
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Main Authors: | , , |
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Format: | Article |
Language: | English |
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Faculty of Resource Science and Technology
2011
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Online Access: | http://ir.unimas.my/id/eprint/31740/1/HOT%20PHENOL%20EXTRACTION%20OF%20TOTAL%20RNA%20-%20Copy.pdf http://ir.unimas.my/id/eprint/31740/ http://publisher.unimas.my/ojs/index.php/BJRST/article/view/264 |
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Institution: | Universiti Malaysia Sarawak |
Language: | English |