Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa)
A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the...
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Rapid Science Publishers
1998
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Online Access: | http://ir.unimas.my/id/eprint/33146/1/Use%20of%20randomly%20ampli%C3%86ed%20polymorphic%20DNAanalysis%20to%20differentiate%20isolates%20ofVibrio%20para-haemolyticusfrom%20cockles%20%28Anadara%20granosa%29_pdf.pdf http://ir.unimas.my/id/eprint/33146/ |
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my.unimas.ir.331462020-12-02T03:18:48Z http://ir.unimas.my/id/eprint/33146/ Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) Radu, Son El-Hadi, Nasreldin Samuel, Lihan Gulam, Rusul Nimita, F. Q Science (General) A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. Rapid Science Publishers 1998 Article PeerReviewed text en http://ir.unimas.my/id/eprint/33146/1/Use%20of%20randomly%20ampli%C3%86ed%20polymorphic%20DNAanalysis%20to%20differentiate%20isolates%20ofVibrio%20para-haemolyticusfrom%20cockles%20%28Anadara%20granosa%29_pdf.pdf Radu, Son and El-Hadi, Nasreldin and Samuel, Lihan and Gulam, Rusul and Nimita, F. (1998) Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa). World Journal of Microbiology and Biotechnology, 14. pp. 895-901. |
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Q Science (General) Radu, Son El-Hadi, Nasreldin Samuel, Lihan Gulam, Rusul Nimita, F. Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) |
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A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. |
format |
Article |
author |
Radu, Son El-Hadi, Nasreldin Samuel, Lihan Gulam, Rusul Nimita, F. |
author_facet |
Radu, Son El-Hadi, Nasreldin Samuel, Lihan Gulam, Rusul Nimita, F. |
author_sort |
Radu, Son |
title |
Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) |
title_short |
Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) |
title_full |
Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) |
title_fullStr |
Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) |
title_full_unstemmed |
Use of randomly ampliÆed polymorphic DNA analysis to differentiate isolates of Vibrio para-haemolyticus from cockles (Anadara granosa) |
title_sort |
use of randomly ampliæed polymorphic dna analysis to differentiate isolates of vibrio para-haemolyticus from cockles (anadara granosa) |
publisher |
Rapid Science Publishers |
publishDate |
1998 |
url |
http://ir.unimas.my/id/eprint/33146/1/Use%20of%20randomly%20ampli%C3%86ed%20polymorphic%20DNAanalysis%20to%20differentiate%20isolates%20ofVibrio%20para-haemolyticusfrom%20cockles%20%28Anadara%20granosa%29_pdf.pdf http://ir.unimas.my/id/eprint/33146/ |
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