Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

• Main Authors: Akinsola, Rasaq Olajide, Lee, Choon-Weng, Sim, Edmund U. H., Narayanan, Kumaran
• Format: Article
• Language: English
• Published: Elsevier 2021
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/34011/1/Inhibition%20of%20lysosomal%20vacuolar%20proton%20pump%20down-regulates%20cellular%20acidification%20and%20enhances%20E.%20coli%20bactofection%20efficiency.pdf; http://ir.unimas.my/id/eprint/34011/; https://www.journals.elsevier.com/analytical-biochemistry/
• Institution: Universiti Malaysia Sarawak

Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during deliver...