Effect of pH for One-step Enzymatic Hydrolysis of Sago Waste for Glucose Production

• Main Author: Muhamad Iqbal, Bahri
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2014
• Subjects: S Agriculture (General); SB Plant culture
• Online Access: http://ir.unimas.my/id/eprint/34372/4/M%20Iqbal%20ft.pdf; http://ir.unimas.my/id/eprint/34372/
• Institution: Universiti Malaysia Sarawak

Sago waste from sago palm (Metroxylon sagu) is a source of glucose because it still contain starch that are trapped inside the fibre and need pretreatment to get the starch out. The objectives of this project are to select the suitable pH for one-step enzymatic hydrolysis of sago waste which can obtain higher glucose concentration and to determine the optimum reaction time of one-step enzymatic hydrolysis of sago waste. One-step enzymatic hydrolysis was the addition of enzymes into a vessel in one time. In this project, the liquefaction and saccharification steps were combined while the enzymes involve were Liquozyme SC DS, Spirizyme Fuel and Celluclast. The hydrolysis was done using three different pH (4.0, 4.5, and 5.0) of KH2PO4 buffer solution. The hydrolysis were conducted for 30 hours under temperature 60°C. From three pH, pH 4.5 yield the highest glucose concentration that was 23.99 g. For the lowest glucose concentration yield was pH 5.0 with 17.55 g. An amount of 21.28 g glucose was obtained using pH 4.0. Meanwhile, the percentage of glucose recovery for pH 4.0 was 70.93 %, pH 4.5 was 79.95 % and 58.49 % for pH 5.0. The hydrolysis was stopped at 30 hours because the conversion of sago waste to glucose start to decrease. Therefore, pH 4.5 is selected as the best pH for one-step enzymatic hydrolysis to obtain high glucose concentration.