Isolation and Characterisation of Salmonella spp. from Water in Aquaculture Farms
Aquaculture has become one of the world largest industries due to high demand of aquaculture products as a part of food supply. In Malaysia, aquaculture especially in freshwater pond aquaculture has developed quickly and become an important activity. In spite of that, chloramphenicol, one of the b...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/35161/1/Scholastica%20Ramih%2824pgs%29.pdf http://ir.unimas.my/id/eprint/35161/4/Scholastica%20Ramih%20ft.pdf http://ir.unimas.my/id/eprint/35161/ |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English English |
| Summary: | Aquaculture has become one of the world largest industries due to high demand of aquaculture products as
a part of food supply. In Malaysia, aquaculture especially in freshwater pond aquaculture has developed
quickly and become an important activity. In spite of that, chloramphenicol, one of the banned antibiotics
in aquaculture, is illegal used of this antibiotic become a major concern due to the safety of consumer
which depends on the development of drug resistant strains in bacterial Salmonella spp. Due to the
circumstances stated above, the objectives of this study were to isolate the Salmonella spp. from water in
both traditional and commercial farm, to detect the chloramphenicol resistance gene in Salmonella spp.
isolated from both traditional and commercial farm and to determine the susceptibility of Salmonella spp.
towards chloramphenicol by using antibiotic susceptibility test. Salmonella spp. that isolated from
aquaculture farms were grown selectively on XLD agar and assessed for antibiotic susceptibility test using
chloramphenicol disks. The corresponding chloramphenicol resistance genes in the isolates were detected
by specific PCR which targeted the catP gene. The chloramphenicol susceptibility test showed that 15
(79%) isolates were susceptible towards chloramphenicol, while 4 (21 %) isolates were intermediate
resistance to chloramphenicol. Based on this study, 17 out of 20 isolates including the Salmonella spp.
control were detected with catP gene. These findings provided information on chloramphenicol resistance
profiles of Salmonella spp. in aquaculture farms to control the usage of antibiotics in aquaculture |
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