Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia
The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown tha...
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Online Access: | http://ir.unimas.my/id/eprint/35883/1/zoonotic2.pdf http://ir.unimas.my/id/eprint/35883/ https://www.mdpi.com/2076-2615/10/12/2367 https://doi.org/10.3390/ani10122367 |
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my.unimas.ir.358832023-03-30T06:14:45Z http://ir.unimas.my/id/eprint/35883/ Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia Ain-Najwa, Mohd Yuseri Yasmin, Abd Rahaman Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni Omar Natasha, Jafar Ali Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd-Lutfi J. Rovie-Ryan, Jeffrine QL Zoology The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown that 1:7 and 1:6 Malaysian wild birds are WNV antibody and RNA positive, respectively, and bats in North America may not be susceptible to the WNV infection. This study was conducted to determine the status of WNV in Malaysian macaques and bats found in mangrove forests and caves, respectively. Archive sera and oropharyngeal swabs from long-tailed macaques were subjected to the antibody detection using WNV competitive enzyme-linked immunosorbent assay (c-ELISA) and WNV RNA using RT-PCR, respectively, while the archive oropharyngeal and rectal swabs from bats were subjected to RT-PCR without serological analysis due to the unavailability of serum samples. The analysis revealed a WNV seropositivity of 29.63% (24/81) and none of the macaques were positive for WNV RNA. Meanwhile, 12.2% (5/41) of the bats from Pteropodidae, Emballonuridae, and Rhinolophidae families tested positive for WNV RNA. Here, we show a high WNV antibody prevalence in macaques and a moderate WNV RNA in various Malaysian bat species, suggesting that WNV circulates through Malaysian wild animals and Malaysian bat species may be susceptible to the WNV infection MDPI 2020-12-10 Article PeerReviewed text en http://ir.unimas.my/id/eprint/35883/1/zoonotic2.pdf Ain-Najwa, Mohd Yuseri and Yasmin, Abd Rahaman and Arshad, Siti Suri and Omar, Abdul Rahman and Abu, Jalila and Kumar, Kiven and Mohammed, Hussni Omar and Natasha, Jafar Ali and Mohammed, Mohammed Nma and Bande, Faruku and Abdullah, Mohd-Lutfi and J. Rovie-Ryan, Jeffrine (2020) Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia. Animals, 10 (12). pp. 1-13. ISSN 2076-2615 https://www.mdpi.com/2076-2615/10/12/2367 https://doi.org/10.3390/ani10122367 |
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QL Zoology Ain-Najwa, Mohd Yuseri Yasmin, Abd Rahaman Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni Omar Natasha, Jafar Ali Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd-Lutfi J. Rovie-Ryan, Jeffrine Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia |
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The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown that 1:7 and 1:6 Malaysian wild birds are WNV antibody and RNA positive, respectively, and bats in North America may not be susceptible to the WNV infection. This study was conducted to determine the status of WNV in Malaysian macaques and bats found in mangrove forests and caves, respectively. Archive sera and oropharyngeal swabs from long-tailed macaques were subjected to the antibody detection using WNV competitive enzyme-linked immunosorbent assay (c-ELISA) and WNV RNA using RT-PCR, respectively, while the archive oropharyngeal and rectal swabs from bats were subjected to RT-PCR without serological analysis due to the unavailability of serum samples. The analysis revealed a WNV seropositivity of 29.63% (24/81) and none of the macaques were positive for WNV RNA. Meanwhile, 12.2% (5/41) of the bats from Pteropodidae, Emballonuridae, and Rhinolophidae families tested positive for WNV RNA. Here, we show a high WNV antibody prevalence in macaques and a moderate WNV RNA in various Malaysian bat species, suggesting that WNV circulates through Malaysian wild animals and Malaysian bat species may be susceptible to the WNV infection |
format |
Article |
author |
Ain-Najwa, Mohd Yuseri Yasmin, Abd Rahaman Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni Omar Natasha, Jafar Ali Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd-Lutfi J. Rovie-Ryan, Jeffrine |
author_facet |
Ain-Najwa, Mohd Yuseri Yasmin, Abd Rahaman Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni Omar Natasha, Jafar Ali Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd-Lutfi J. Rovie-Ryan, Jeffrine |
author_sort |
Ain-Najwa, Mohd Yuseri |
title |
Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia |
title_short |
Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia |
title_full |
Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia |
title_fullStr |
Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia |
title_full_unstemmed |
Exposure to Zoonotic West Nile Virus in Long-Tailed Macaques and Bats in Peninsular Malaysia |
title_sort |
exposure to zoonotic west nile virus in long-tailed macaques and bats in peninsular malaysia |
publisher |
MDPI |
publishDate |
2020 |
url |
http://ir.unimas.my/id/eprint/35883/1/zoonotic2.pdf http://ir.unimas.my/id/eprint/35883/ https://www.mdpi.com/2076-2615/10/12/2367 https://doi.org/10.3390/ani10122367 |
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1762396691788464128 |